摘要
目的 探讨用ELISA检测华支睾吸虫病患者血清中特异性IgG4 的诊断价值。 方法 用华支睾吸虫成虫可溶性抗原进行ELISA分别检测华支睾吸虫病患者 (76例 )血清中的特异性IgG和IgG4 抗体及日本血吸虫病、并殖吸虫病、囊尾蚴病患者 (分别为 63、3 5、41例 )和健康者 (65人 )血清中的交叉抗体。 结果 检测 76例华支睾吸虫病患者血清中IgG4 的检出率为 93 .42 % ,检测 65例健康者血清中的IgG4 均为阴性 ,分别与检测IgG结果比较差异均无显著性意义 (P均 >0 .0 5 ) ;与其它寄生虫病的交叉反应率 ,IgG4 显著低于IgG ;诊断效率及阳性、阴性预告值分别为 96.45 %、10 0 %和 92 .85 %。 结论 用ELISA检测华支睾吸虫病患者血清中特异性IgG4 具有较高的诊断应用价值。
Objective To prove the value of ELISA in detecting serum IgG 4 in the diagnosis of clonorchiasis. Methods ELISA was used to detect the specific IgG and IgG 4 antibodies in sera from the patients with clonorchiasis and other parasitic diseases and from healthy persons. Results The sensitivity and specificity of IgG 4 antibodies detected by ELISA in sera from the patients with clonorchiasis showed no significant difference with that of IgG detected. The cross reaction rate of IgG 4 in sera from patients infected with other parasites was significantly lower than that of IgG detection. The diagnostic efficiency and the positive and negative predictive values were 96.45%, 100% and 92.85%, respectively. Conclusion The specific IgG 4 detected by ELISA showed the same sensitivity and specificity as IgG in diagnosing clonorchiasis and less cross reaction with sera from the patients infected with other parasites. Therefore, the specific IgG 4 detection by ELISA had higher diagnostic value than IgG detection in diagnosing clonorchiasis.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2002年第5期289-291,共3页
Chinese Journal of Parasitology and Parasitic Diseases
