摘要
目的：探讨日本血吸虫感染中循环抗原动态变化及其机制。方法：感染日本血吸虫的ＢＡＬＢ／ｃ小鼠淋巴结Ｂ细胞和小鼠骨髓瘤细胞（ＳＰ２／０株）融合，制备抗血吸虫循环抗原不同表位的单克隆抗体组合后为探针，检测日本血吸虫病患者和正常人血清；晚期血吸虫病患者脾血和外周血；感染日本血吸虫兔和小鼠的门脉血和外周血。结果：获得９种抗日本血吸虫单克隆抗体，将其中抗不同抗原表位的单抗优化组合后使用，检测抗原的敏感性可提高４－８倍。用组合单抗建立的夹心ＥＬＩＳＡ和ｄｏｔ－ＥＬＩＳＡ检测慢性血吸虫病的敏感性分别为７４％和８４．２％，特异性分别为９６．１％和９５．７％。通过小鼠和家兔门脉血和外周血以及人的脾血和外周血循环抗原的比较研究表明，门脉血中的抗原水平明显高于外周血。结论：用组合单抗较用一种单抗提高检出率。ｄｏｔ－ＥＬＩＳＡ检测的不仅是循环抗原，同时还检测到免疫复合物，因此影响疗效考核。
AIM: To observe the dynamic changes of circulating antigen in Schistosoma japonicum infection and its mechenism. METHOD: After fusion of B lymphocytes from mice infected with S.japonicum and mouse myeloma cell(SP2/0 strain), McAbs against different schistosomal antigenic epitopes were combined as probes to detect sera from schistosomiasis cases, splenic blood and peripheral blood of advanced schistosomiasis cases, portal vein blood and peripheral blood of rabbits and mice infected with S.japonicum. RESULTS: Nine monoclonal antibodies against schistosome antigen were detected by IFA . When these McAbs against different antigenic epitopes were used, in combination the sensitivity of antigen detection could be raised by 4-8 times. established on the basis of compounded McAbs. When sandwich ELISA and dot ELISA were used for detection of chronic schistosomiasis, the sensitivity was 74% and 84 2%, the specificity was 96 1% and 95 7%, respectively. The circulating antigen level were significantly higher in portal vein blood and splenic blood than in peripheral blood. CONCLUSION: McAbs against different schistosomal antigens used in combination in sandwich ELIAS could enhance the sensitivity of antigen detection. Not only circulating antigen but also immune complex of schistosomes were detected by dot ELISA, so it might interfere with judgement of curative effect.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1996年第1期5-10,共6页
Chinese Journal of Parasitology and Parasitic Diseases
基金
国家自然科学基金
关键词
日本血吸虫
单克隆抗体
循环抗原
Schistosoma japonicum, monoclonal antibody, circulating antigen
