摘要
目的:探讨姜黄素对骨肉瘤细胞增殖、凋亡的影响及作用机制。方法:选用骨肉瘤MG-63细胞株,分别采用不同浓度的姜黄素进行处理。采用MTT比色法检测骨肉瘤MG-63细胞增殖抑制率,采用流式细胞仪检测骨肉瘤MG-63细胞凋亡情况,采用Western Blot法检测骨肉瘤MG-63细胞凋亡相关蛋白表达水平。结果:①干预12、24、48 h后,与对照组比较,姜黄素各浓度组骨肉瘤MG-63细胞增殖抑制率较高,且姜黄素5μmol/L组<10μmol/L组<15μmol/L组及20μmol/L组,差异有统计学意义(P<0.05)。②干预48 h后,与对照组比较,姜黄素各浓度组骨肉瘤MG-63细胞凋亡率较高,且姜黄素5μmol/L组<10μmol/L组<15μmol/L组,差异有统计学意义(P<0.05)。③与对照组比较,黄素各浓度组骨肉瘤MG-63细胞P53、B细胞淋巴瘤/白血病-2(Bcl-2)、Caspase-9前体(proCaspase-9)蛋白表达水平较低,且姜黄素5μmol/L组>10μmol/L组>15μmol/L组,差异有统计学意义(P<0.05)。④与对照组比较,姜黄素各浓度组骨肉瘤MG-63细胞Bcl相关蛋白(Bax)、细胞色素C(Cyt-C)、天冬氨酸特异性半胱氨酸蛋白酶-3(Caspase-3)蛋白表达水平较高,且姜黄素5μmol/L组<10μmol/L组<15μmol/L组,差异有统计学意义(P<0.05)。结论:姜黄素能剂量依赖性的抑制骨肉瘤MG-63细胞增殖,并促进其凋亡,其作用可能与下调P53、Bcl-2、proCaspase-9蛋白表达,上调Bax、Cyt-C、Caspase-3蛋白表达,激活线粒体凋亡途径有关。
Objectives:To analysis the effects and mechanism of anticancer drug curcumin on proliferation and apoptosis of osteosarcoma cells. Methods:Osteosarcoma MG-63 cells were selected and treated with different concentrations of curcumin. The osteosarcoma MG-63 cells proliferation inhibition rate was detected by MTT colorimetric method,apoptosis of osteosarcoma MG-63 cells was detected by flow cytometry,the expression levels of apoptosis related protein were detected by Western Blot method in osteosarcoma MG-63 cells. Results:(1) After the intervention of 12 h,24 h and 48 h,compared with control group,the proliferation inhibition rate of osteosarcoma MG-63 cells were higher in each concentration of curcumin group,and curcumin 5 μmol/L group<10 μmol/L group<15 μmol/L group and 20 μmol/L group,the differences were statistically significant(P<0.05).(2)After the intervention of 48 h,compared with control group,the apoptosis rate of osteosarcoma MG-63 cells were higher in each concentration of curcumin group,and curcumin 5 μmol/L group<10 μmol/L group<15 μmol/L group,the differences were statistically significant(P<0.05).(3)Compared with control group,the expression of P53,B cell lymphoma/leukemia-2(Bcl-2),Caspase-9 precursor(proCaspase-9)protein in osteosarcoma MG-63 cells were lower in each concentration of curcumin group,and curcumin 5 μmol/L group>10 μmol/L group>15 μmol/L group,the differences were statistically significant(P<0.05).(4)Compared with control group,the expression of Bcl related protein(Bax),cytochrome C(Cyt-C),aspartate specific cysteine protease-3(Caspase-3)protein in osteosarcoma MG-63 cells were higher in each concentration of curcumin group,and curcumin 5 μmol/L group<10 μmol/L group<15 μmol/L group,the differences were statistically significant(P<0.05). Conclusion:Anticancer drug curcumin can inhibit the proliferation and promote apoptosis of osteosarcoma MG-63 cells in a dose-dependent manner,the effects may be related to down-regulation of P53,Bcl-2,proCaspase-9 protein expression,up-regulation of Bax,Cyt-C,Caspase-3 protein expression,and the activation of mitochondrial apoptosis pathway.
作者
吴峻
WU Jun(Baoji Vocational Technology College,Baoji 721000,Shaanxi,China)
出处
《辽宁中医药大学学报》
CAS
2019年第5期37-41,共5页
Journal of Liaoning University of Traditional Chinese Medicine
基金
陕西省科技攻关计划项目(2015K09-16)
关键词
姜黄素
骨肉瘤细胞
细胞增殖
细胞凋亡
机制
curcumin
osteosarcoma cells
cell proliferation
apoptosis
mechanism
