摘要
目的对32种中草药进行初步抗骨肉瘤的体外筛选实验,为中药标准化和治疗骨肉瘤提供依据。方法应用MTT法测定各中药提取物对U2OS细胞株增殖活性的影响,检测其抑制骨肉瘤细胞的剂量效应关系;应用形态学观察、FCM及AnnexinV法测定筛选出的中药水提取物对U2OS细胞的凋亡作用。结果32种中草药提取物中蟾酥、牛胆粉等对骨肉瘤U2OS细胞株具有增殖抑制作用,深入研究表明蟾酥、牛胆粉水提取物对U2OS细胞具有促进凋亡作用,其中以蟾酥促进凋亡作用最为明显。结论通过对32种中草药进行筛选,发现蟾酥、牛胆粉等对骨肉瘤细胞株U2OS的增殖有抑制作用,为进一步开展抗骨肉瘤中草药有效成分的筛选及体内动物研究提供实验依据。
Objective To test 32 traditional Chinese medicinal herbs with effects against osteosarcoma in vitro. Methods U2OS human osteosarcoma cell line was treated with the extracts of the Chinese herbs at various concentrations. The changes in cell proliferation in response to the treatment were examined by MTT assay, and the effects of these extracts against human osteosarcoma growth were compared. Morphological observation, flow cytometry and Annexin V were employed to detect the cell apoptosis aider the treatments. Results According to the results of MTT assay, several of the 32 Chinese herbs, especially Venenum Bufonis and oxgall powder, were identified to produce growth inhibition against U2OS cells. Further study of the aqueous extracts of Venenum Bufonis and oxgall powder demonstrated their effects in inducing U2OS cell apoptosis, and Venenum Bufoni, s showed the strongest effect. In spite of the obvious growth inhibitory effect of oxgall powder, its extract induced cell apoptosis only at high concentrations. Conclusion Several of the traditional Chinese herbs, especially Venenum Bufonis and oxgall powder, may inhibit the growth of U2OS cell line, and the results of this study pave the way for further identification of the effective components in the herbs that inhibit osteosarcoma growth both in vivo and in vitro.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2006年第9期1293-1296,共4页
Journal of Southern Medical University
基金
国家教委归国人员启动基金(1999747)
广东省自然科学基金(4009423)~~
关键词
中草药
蟾酥
牛胆粉
骨肉瘤
凋亡
Chinese herbs
Venenum Bufonis
Ox-bile powder
osteosarcoma
apoptosis
