摘要
采用茶汤有机试剂萃取分离,并分别经过反相C_(18)柱层析纯化,制备出乙酸乙酯层、正丁醇层和酸性正丁醇层三部分茶红素。根据紫外可见光波扫描计算出色价,并研究了茶红素在不同条件下的稳定性。结果表明,30%、50%和70%甲醇梯度洗脱,纯化后3类茶红素液相检测发现无咖啡碱和茶黄素,仅有少量儿茶素杂质。纯化后色素色价分别为乙酸乙酯层128.04,正丁醇层91.04,酸性正丁醇层76.16。遮光处理,p H=3酸性条件,45℃以下贮藏,有利于茶红素的稳定性。
In this study,thearubigins were extracted by organic extraction,and purified by reversed-phase C18column chromatography to obtain three types of thearubigins,namely ethyl acetate,n-butanol and acid n-butanolextracts.The stability of thearubigins under different conditions was analyzed by scanning UV-Vis absorptionwavelength based on color difference.The results showed that there was no caffeine and theaflavins,but only a smallamount of catechins were detected in the three kinds of thearubigins after30%,50%and70%methanol gradientelution.The color values of thearubigins after purification were128.04for ethyl acetate extracts,91.04for n-butanolextracts and76.16for acidic n-butanol extracts.Shading treatment,pH=3acidic conditions and temperature below45℃will facilitate the stability of thearubigins.
作者
李山
陈小强
滑金杰
李佳
董春旺
王银诚
江用文
袁海波
LI Shan;CHEN Xiaoqiang;HUA Jinjie;LI Jia;DONG Chunwang;WANG Yincheng;JIANG Yongwen;YUAN Haibo(Tea Research Institute, Chinese Academy of Agricultural Sciences, Engineering Research Center for Tea Processing, Hangzhou 31 0008,China;School of Bioengineering and Food, Hubei University of Technology, Wuhan 430068, China)
出处
《茶叶科学》
CAS
CSCD
北大核心
2017年第2期201-210,共10页
Journal of Tea Science
基金
基金项目:国家茶叶产业技术体系红茶加工岗位(CARS-23)
中国农业科学院科技创新工程(CAAS-ASTIP-TRICAAS)
浙江省农产品化学与加工技术重点实验室/浙江省农业生物资源生化制造协同创新中心(2016KF0012)
关键词
茶红素
柱层析
色价
稳定性
thearubigins
chromatography
color values
stability
