摘要
建立了高效阴离子交换色谱-脉冲安培检测法定量分析低聚异麦芽糖的方法。采用CarboPakTM PA10色谱柱,配合安培检测器,以NaOH及醋酸钠为洗脱剂。采用此方法不仅一次性实现了低聚异麦芽糖常规组分中葡萄糖、麦芽糖、麦芽三糖、异麦芽糖、异麦芽三糖,潘糖的有效分离,也实现了异麦芽四糖、异麦芽五糖、异麦芽六糖、异麦芽七糖、海藻糖、麦芽酮糖、曲二糖、黑曲霉糖高聚合度糖及二糖同分异构体间的分离及检测。以不同浓度的标准糖混合溶液建立了校正曲线,此方法中,各组分在0.032～25.975 mg/L间具有良好的线性关系,各物质的检测限和定量限分别在0.008～0.022 mg/L和0.027～0.073 mg/L,样品加标回收率为82.02%～116.37%。
A one-step method for quantitatively determination of isomahooligosaccharides （IMO） was developed using high performance anion exchange chromatography coupled with pulsed amperometric detection （HPAEC-PAD）. The method was built on a CarboPakTM PAl0 column using NaOH and NaAC as eluents. Using this method, besides conventional components of isomahose, isomaltotriose, panose and some saccharides with higher DP were identified from IMO syrup, other transglycosylation saccharides such as trehalose, kojibiose, nigerose and maltulose were also detected from the syrup. Calibration was carried out by dissolving 15 kinds of standard samples containing glucose, fructose, maltose, maltotriose, isomaltose, isomaltotriose, isomaltotetraose, isomaltopentaose, isomaltohexaose, iso- maltoheptaose, panose, trehalose, maltulose, kojibiose, and nigerose into a mixed solution. The standard solution was diluted to a calibration range from 0. 032 to 25. 975 mg/L. The calibration curves showed good linearity of IMO within this range. The detection limits （LODs） and the quatification limits （LQD） were 0. 008 - 0. 022 mg/L and 0. 027 -0. 073 mg/L respectively, and the relative standard deviations were 82.02% - 116.37%. This method was good and sensitive in the quantitative analysis of IMO.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2012年第7期144-147,共4页
Food and Fermentation Industries
