摘要
应用荧光光谱法研究了水溶液中除草剂莠去津与过氧化氢酶分子间的相互作用 .结果表明 ,除草剂对过氧化氢酶的荧光有较强的猝灭作用 ,且静态猝灭是引起 CAT荧光猝灭的主要原因 .从荧光猝灭结果求出除草剂和 CAT的结合常数及结合位点数分别为 K=1.35× 10 5L/mol,n=1.17.并依据能量转移机制 ,求出了莠去津和 CAT相互结合时 ,其给体 -受体间距 r为 0 .15 9nm.由此可见 ,莠去津与过氧化氢酶的相互结合作用以静态猝灭过程为主 ,且其猝灭机制是通过能量转移产生的 .推测出莠去津与CAT的 16位 Tyr发生结合作用 .
The binding of atrazine to catalase(CAT) in aqueous solution was studied with fluorospectroscopic method. The results show that atrazine has a strong ability to quench the CAT fluorescence mainly through a static quench procedure. The binding constant K and the number of binding site n were calculated according to the fluorescence quenching results. Based on the mechanism of energy transfer, the donor-acceptor distance of the herbicide and CAT was calculated as 0.159. It is inferred that the binding site of CAT to atrazine is Tyr-16.
出处
《浙江大学学报（理学版）》
CAS
CSCD
2002年第5期542-547,共6页
Journal of Zhejiang University（Science Edition）
基金
国家自然科学基金资助项目 (396 70 4 2 0 )
