摘要
对荧光共振能量转移（ＦＲＥＴ）技术及其应用进行了较全面的综述，介绍了Ｆｏｒｓｔｅｒ原理，ＦＲＥＴ实验技术（包括给体－受体对的选择、标记方法及能量转移效率的测定），及其在生物大分子结构与功能研究、免疫分析和核酸杂交分析等几方面的应用，并对其将来的发展作出了一些评价与展望。
Application of fluorescence resonance energy transfer (FRET) technique in bio- analysis are reviewed with 53 references. The review begins with a summary of Forster' S theory, and some of the most commonly used experimental strategy, including the selection of donor-acceptor couple, labeling methods and the detection of energy transfer efficiency. The application of FRET in the study of the structure and function of biological macro- molecules, in immunoassay and in DNA hybridization assay are discussed in subsequent sec- tions. Finally, the prospects for the future application of this technique is conducted.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1998年第4期477-484,共8页
Chinese Journal of Analytical Chemistry
关键词
荧光共振
能量转移
生物大分子
分析
FRET
结构
Fluorescence resonance energy transfer, bio-macromolecule, structural analysis, immunoassay, deoxyribonucleic acid hybridization assay, review
