摘要
目的 应用DiI(1,1′ dioctadecyl 3,3,3′,3′ tetramethylin docarbocyanineperchlorate ,一种羰花青染料 )染色晶体研究人胚胎视网膜、外侧膝状体、上丘及视皮质间连接的形态学特征和动态发育过程。方法 对不同胎龄的 7个胎儿眼球标本进行灌注固定后 ,分别于标本的视束、上丘臂和视皮质下板层植入DiI染色晶体 ,以标记来自视网膜和外侧膝状体的轴突。于室温下放置 4～ 10周 ,等待DiI染色晶体扩散 ,再根据神经走向切片 ,通过激光共焦扫描显微镜观察并记录植入DiI染色晶体 12～ 2 8周的结果。结果 植入DiI染色晶体 12周时 ,胎儿标本的视网膜神经纤维投射已经到达外侧膝状体 ,但尚未出现分层现象 ;视网膜神经投射也已到达上丘 ,纤维位于上丘臂的背侧 ;视皮质下已出现板层结构。植入 2 2周时视皮质下仍存在板层结构。结论 植入DiI染色晶体 12周前 ,视网膜神经纤维投射已到达外侧膝状体和上丘 ,视皮质下的板层也已经形成 ,2 2周后消失。DiI染色技术能有效地用于研究胚胎期视神经的连续发育过程。
Objective To reveal the morphological features and dynamic processes of the development of inter connections in the retina, lateral geniculate nucleus (LGN), superior colliculus (SC) and visual cortex (VC) in human fetal life by using a fluorescent tracer, 1,1′ dioctadecyl 3,3,3′,3′ tetramethylin docarbocyanine perchlorate (DiI). Methods DiI was embedded into the optic tract, brachium of superior colliculus and subplate of visual cortex in fixed postmortem human tissues of 7 fetuses. The tissue was incubated at 37℃ for 4 to 10 weeks (ws). After DiI had satisfactorily diffused via axons of the visual system, the tissue was sectioned, mounted and observed under a confocal laser scanning microscope. Results In 12 week fetus, retinogeniculate axon has already reached LGN, but there was no cellular lamination. After embedment, axons from retina arrived at SC, and the fibers were distributed along the dorsal part of the SC. At 12 and 22ws, there were subplates under visual cortex. Conclusion The retinogeniculate axon reaches LGN before 12ws and forms cellular lamination after 12ws. The axon from retina reaches SC before 12ws. The subplate under visual cortex forms before 12ws and disappears after 22ws. DiI can be easily and effectively used to label the axon of visual system of human fetus to study the prenatal development of human visual pathway.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
2002年第9期517-519,共3页
Chinese Journal of Ophthalmology
基金
国家自然科学基金资助项目 (3 0 14 0 0 16)
浙江省自然科学基金资助项目 (3 0 10 3 7)
教育部科学技术研究重点基金资助项目 (0 10 46)
关键词
视神经
胚胎
甲胺类
Optic nerve
Embryo
Methylamines
