摘要
建立了人胚视网膜神经层分散细胞培养方法，并应用相差显微镜和免疫组织化学方法对视网膜神经元的体外发育进行了初步研究。结果表明：①选择３～４个月的人胚胎视网膜为培养材料比较适合；②体外培养的视网膜神经元经历了贴壁、重聚、迁移、分化和相互接触的过程；③免疫组化显示神经元呈ＮＳＥ阳性，非神经元细胞呈阴性。
A monolayer culture procedure was developed for study on oell development and interaotion during human retina histogeneais. Oells from trypsinized 3￣4embryonic month hnman retina reaggregated within honrs into olusters which later developed interconnectiongs. Neurons and non-neuronal cells were identified by inverted phase contrast microseope and ABC immunochemistry with antibody against neuron speeific enolase. Based on morphology, neurons could be divided into two main groups: photoreceptor-like neurons and other neurons including bipolar cell, ganglion cell and so forth.
出处
《上海医科大学学报》
CSCD
1995年第1期1-4,共4页
Journal of Fudan University(Medical Science)
基金
国家自然科学基金
关键词
人胎
视网膜
细胞培养
免疫细胞化学
human fetus
retina
cell culture
development
immunocytochemistry
