摘要
目的：建立Sysmex XE-5000血液分析仪检测健康成人静脉血血小板五参数（PLT、PCT、MPV、PDW、P-LCR）的参考区间,探讨适合该实验室的生物参考区间.方法：参照美国临床和实验室标准协会（NCCLS）C28-A2文件推荐方法,用Sysmex XE-5000血液分析仪对1452例健康成人静脉血的血小板五参数进行检测,SPSS19.0对结果进行统计学处理.五参数均为非正态性分布,采用百分位数P2.5～P97.5作为生物参考区间.男女组间对比采用非参数秩和检验.结果：PLT、PCT在不同性别间差异有统计学意义（P〈0.05）,应作性别分组处理.MPV、PDW、P-LCR在不同性别间,差异无统计学意（P〉0.05）,可以合并参考区间.PLT与PCT有显著性相关（r=0.923）,MPV、PDW、P-LCR三者间相关性显著.新建立的生物参考区间为：PLT男（×109/L）123.15～302.70、 女（×109/L）143.20～349.00;PCT（％）男13.00～30.00、 女15.00～34.00;MPV（fL）8.90～12.47;PDW（％）9.40～16.56;P-LCR（％）15.72～45.31.随机抽取194例健康体检职工对新建参考区间进行验证,PLT（男性）2.89％落在区间外,PCT（男性）1.92％落在区间外.MPV（女性）、PDW（女性）、P-LCR（女性）各2.22％落在区间外.结论：PLT、PCT参考区间与性别有关,MPV、PDW、P-LCR参考区间与性别无关.新建立的参考区间适用于该实验室,为临床诊疗工作提供了更有意义的参考数据.
Objective： To establish the reference reference interval of the five parameters （PLT, PCT, MPV, PDW and P-LCR） of healthy adults blood platelets by Sysmex XE-5000 blood analyzer, and to explore the biological reference interval that suitable for the laboratory. Methods： According to the recommendation method of the C28-A2 document of the American Association for clinical and laboratory standards （NCCLS） , the five parameters of blood platelets in 1452 healthy adults were detected by Sysmex XE-5000 blood analyzer, and the results were statistically treated by SPSS19.0. The five parameters are non normal distribution, and percentile P25-P975 is used as biological reference interval. Non - parameter rank sum test was used in the comparison between male and famale groups. Results： The difference between PLT and PCT was statistically significant （P〈0.05）, and should be treated by gender. There was no significant difference in MPV, PDW and P-LCR between genders （P〉0.05） , and the reference interval could be combined. There was a significant correlation between PLT and PCT （r=0.923）. MPV, PDW and P-LCR were significantly correlated. The newly established biological reference ranges are： PLT male （×109/L） 123.15-302.70, female （×109/L） 14320-349.00; PCT （0/0） male 13.00-30.00, female 15.00-34.00; MPV （fL） 8.90- 12.47; PDW （%） 9.40-16.56; P-LCR （0/6） 15.72-45.31. 194 healthy persons were randomly selected to verify the new reference interval. PLT （male, 2.89%） and PCT （male, 1.92%） drooped outside the interval. The rate in either MPV （female） , PDW （female） or P-LCR （female） was 2.22% Conclusion： The reference intervals of PLT and PCT were related to gender. The reference intervals ofMPV, PDW and P-LCR were independent of gender. The newly established reference interval is suitable for the laboratory, which could provide more meaningful reference data for clinical diagnosis and treatment.
作者
罗俭权
陈达勇
罗学俭
李倩
LUO Jian-quan;CHEN Da-yong;LUO Xue-jian(Department of Clinical Laboratory,Sihui People＇s Hospital Zhaoqing,Guangdong Zhaoqing 52620)
出处
《医学检验与临床》
2018年第7期24-27,共4页
Medical Laboratory Science and Clinics
