摘要
目的建立武汉地区健康成人荧光法血小板(PLT)计数(简称PLT-F)和PLT相关参数的参考区间。方法选取2021年11月—2023年8月华中科技大学同济医学院附属同济医院≥18岁的健康体检者1067名。采集所有研究对象静脉全血,采用全自动血液分析仪检测平均血小板体积(MPV)、血小板体积分布宽度(PDW)、大血小板比例(P-LCR)、血小板比容(PCT)和未成熟血小板比例(IPF)等PLT参数。比较不同性别和年龄研究对象PLT相关参数差异,并建立相应参考区间。另选取同期华中科技大学同济医学院附属同济医院健康体检者120名,用于验证参考区间。结果不同性别和年龄研究对象IPF、PDW差异无统计学意义(P>0.05),参考区间分别为(0.90~9.86)fL和(9.5~18.1)%;男性MPV和P-LCR参考区间分别为(9.11~12.69)fL和(17.90~46.09)%,女性MPV和P-LCR参考区间分别为(9.27~13.00)fL和(18.05~48.87)%;青年组、中年组和老年组男性PLT-F参考区间分别为(161~384)×10^(9)L^(-1)、(147~374)×10^(9)L^(-1)和(118~366)×10^(9)L^(-1),女性分别为(149~404)×10^(9)L^(-1)、(157~375)×10^(9)L^(-1)和(124~356)×10^(9)L^(-1);青年组、中年组和老年组男性PCT参考区间分别为(0.19~0.36)%、(0.17~0.37)%和(0.15~0.38)%,女性分别为(0.17~0.43)%、(0.18~0.40)%和(0.15~0.36)%。参考区间验证结果显示符合率为100%。结论初步建立了武汉地区成人PLT-F和PLT相关参数的参考区间,可为临床诊疗提供参考。
Objective To establish the reference ranges for fluorescence-based platelet count(PLT-F)and platelet-related parameters for healthy adults in Wuhan.Methods A total of 1067 healthy subjects aged≥18 years who underwent physical examinations at Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology from November 2021 to August 2023 were enrolled.Venous whole blood samples were collected,and the mean platelet volume(MPV),platelet distribution width(PDW),plateletlarger cell ratio(P-LCR),platelet hematocrit(PCT)and immature platelet fraction(IPF)were determined using automatic hematology analyzer.The data were grouped by gender and age for comparison,and the reference ranges were established.Results There was no statistical significance in IPF and PDW between different genders and ages(P>0.05).The reference ranges were(0.90-9.86)fL and(9.5-18.1)%,respectively.The reference ranges of MPV and P-LCR%for males were(9.11-12.69)fL and(17.90-46.09)%,while those for females were(9.27-13.00)fL and(18.05-48.87)%.The reference ranges of PLT-F and PCT for males in the young,middle-aged and elderly groups were(161-384)×10^(9)L^(-1),(147-374)×10^(9)L^(-1) and(118-366)×10^(9)L^(-1),respectively.For females,the reference ranges of PLT-F and PCT were(149-404)×10^(9)L^(-1),(157-375)×10^(9)L^(-1) and(124-356)×10^(9)L^(-1),respectively.The reference ranges of PCT for males in the young,middle-aged and elderly groups were(0.19-0.36)%,(0.17-0.37)%and(0.15-0.38)%,respectively.For females,the reference ranges of PCT were(0.17-0.43)%,(0.18-0.40)%and(0.15-0.36)%,respectively.The reference ranges were verified and showed a 100%accuracy rate.Conclusions The reference ranges for PLT-F and PLT-related parameters for adults in Wuhan have preliminarily been established.
作者
张辉
袁靖雯
张驰
ZHANG Hui;YUAN Jingwen;ZHANG Chi(Department of Clinical Laboratory,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Hubei,China;Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Hubei,China)
出处
《检验医学》
2025年第9期892-896,共5页
Laboratory Medicine
关键词
血小板计数
血小板参数
参考区间
荧光法
Platelet count
Platelet parameter
Reference range
Fluorescence method
