摘要
为探讨精神创伤后应激障碍样大鼠海马区p38丝裂原活化蛋白激酶(p38MAPK)的改变及其意义。采用将Wistar大鼠随机分为4组的方法:空白对照组、PTSD 1 d组、PTSD 4 d组、PTSD 7 d组,每组各8只。PTSD组建立SPS模型,采用旷场实验、Morris水迷宫等方法检验各组大鼠造模前后的行为学指标变化并评估造模效果,采用逆转录酶-多聚酶链反应(RT-PCR)及蛋白质免疫印迹法(Western blot)测定各组大鼠海马组织中p38MAPK的水平变化。结果显示:应激后大鼠海马区p38MAPK水平升高,差异具有统计学意义(P<0.05)。由此可知,创伤后应激障碍大鼠海马区p38MAPK升高,可能与学习及记忆功能受损有关。
To investigate the changes and significance of p38 MAPK in hippocampus of post-traumatic stress rats. Wistar rats were randomly divided into 4 groups： control group, PTSD 1 d group, PTSD 4 d group and PTSD 7 d group, 8 rats in each group. PTSD group was used to establish SPS model. The behavioral indexes of the rats were measured by open field experiment and Morris water maze. The reverse press of p38 MAPK in hippocampus was detected by RT-PCR and Western blot. The results showed that the levels of p38 MAPK in the hippocampus of rats after stress were significantly increased（P0.05）. The conclusion is that the increase of p38 MAPK in the hippocampus of rats with post-traumatic stress disorder may lead to the loss of learning and memory function.
作者
庞雨婷
邢文龙
张桂青
Pang Yuting;Xing Wenlong;Zhang Guiqing(Department of Psychology and Rehabilitation, the First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang 832002, Chin)
出处
《石河子大学学报（自然科学版）》
CAS
2017年第5期580-584,共5页
Journal of Shihezi University(Natural Science)
基金
新疆兵团医药卫生重点领域科技攻关项目(2012BA023)
