摘要
目的探讨创伤后应激障碍(PTSD)大鼠杏仁核PKMζ的表达变化。方法采用单程长时应激(SPS)制备PTSD大鼠模型,将PTSD大鼠分为3组:SPS3d、SPS7d和SPS14d组,正常饲养大鼠作为对照组。取材各组大鼠杏仁核,利用Western blot和免疫荧光染色检测PKMζ蛋白在大鼠杏仁核表达。结果免疫荧光染色结果显示,SPS7d、SPS14d组大鼠杏仁核PKCζ阳性细胞百分数明显多于正常对照组。Western blot结果显示,SPS7d、SPS14d组大鼠杏仁核PKCζ和PKMζ蛋白相对表达水平显著高于正常对照组。结论 PKCζ和PKMζ在PTSD大鼠杏仁核过表达提示其可能参与PTSD的发病过程。
Objective To investigate the expression changes of PKC ζ and PKM ζ in amygdala of posttraumatic stress disorder（PTSD） rats. Methods The PTSD model was established by single prolonged stress paradigm （SPS）. PTSD rats were divided into： SPS3d, SPS7d and SPS14d group, normal fed rats as a control group. The expression of PKCζ was determined by immunofluorescence staining, the expressions of PKC ζ and PKM ζ proteins were detected by Western blot. Results The percentage of PKC ζ positive cells in amygdala was higher in SPS7d and SPS14d groups than in normal control, the relative expression levels of PKC ζ and PKM ζ proteins in amygdala were increased in SPS7d and SPS14d rats than in normal control group. Conclusion The overexpression of PKC ζ and PKM ζ in the amygdala of PTSD rats might be involved in the pathogenesis of PTSD.
出处
《解剖科学进展》
2017年第5期456-458,共3页
Progress of Anatomical Sciences
基金
辽宁省自然科学基金(2015020459
201602825)
