摘要
目的采用复合应激刺激探索建立一种新的创伤后应激障碍(posttraumatic stress disorder,PTSD)大鼠模型,观察其行为学变化。方法 30只成年SD大鼠,随机分为正常对照组、连续单一应激组(single-prolonged stress,SPS)和复合应激组,每组10只。用束缚、电击、力竭游泳复合应激源建立大鼠模型,14 d后利用迷宫、旷场实验检测其行为学变化,评估模型效果。结果在接受复合应激后第14天大体行为学观察、拒俘反射评分、旷场试验、高架十字迷宫以及水迷宫结果表明复合应激组大鼠较正常对照组及SPS组,显示出显著的PTSD样症状行为学改变,活动性降低、焦虑样行为增多、空间记忆能力受损。①复合应激组拒俘反射评分(1.4±1.0)较SPS组及正常对照组显著增加(P<0.05,P<0.01)。②旷场实验:复合应激组跨格次数(27.80±5.55)和站立次数(9.00±2.49)较SPS组及正常对照组显著减少(P<0.05,P<0.01);修饰次数无显著差异。③高架十字迷宫实验:复合应激组进入开放臂次数比例(27.50±1.35)、进入开放臂时间比例(29.62±3.23)以及OE+CE(8.60±3.63)较SPS组及正常对照组显著降低(P<0.05,P<0.01);向下探究次数和站立次数均无显著差异。④水迷宫实验的第1、2天3者无显著差异,第3天和第4天复合应激组(35.67±5.13、25.09±3.55)较SPS组及正常对照组时间显著延长(P<0.05,P<0.01)。水迷宫空间探索实验中,复合应激组进入目标象限次数(5.80±2.34)以及穿越原平台位置次数(3.10±1.79)较SPS组及正常对照组显著减少(P<0.05,P<0.01)。结论通过大鼠行为学检测证实复合应激刺激可成功制备较典型的大鼠PTSD动物模型。
Objective To establish a new rat model of posttraumatic stress disorder（PTSD） with compound stress stimulations.Methods Thirty male adult SD rats were randomly divided into 3 groups： a normal group,a single-prolonged stress（SPS） group and a compound stress group（CS）（n=10）.The CS rats were given compound stress stimulations of restraint,electric shock and exhaustive swimming to establish the PTSD rat model.The Morris water maze test,elevated plus maze（EPM） test and open-field test were used to examine the behavioral changes of the rats after 14 d.Results The rats of the CS group showed significant PTSD-like behavioral changes,lower locomotor activity,more anxiety-like behavior and poorer spatial cognitive ability compared with the normal group and SPS group on the 14th day after stimulation.（1） The scale of rat response to capture significantly increased in the CS group（1.4±1.0） compared with that in the normal group and the SPS group（P0.05,P0.01）.（2） The open-field tests revealed that the times of crossing（27.80±5.55） and rearing（9.00±2.49） of the CS group significantly decreased compared with those of the normal group and the SPS group（P0.01,P0.05）,but there was no significant difference of grooming times among the three groups.（3） The data of EPM test showed that the ratio of open arm entry（OE%）（27.50±1.35）,the ratio of open arm time（OT%）（29.62±3.23） and open arm entry ＋ close arm entry（OE＋CE）（8.60±3.63） significantly decreased in the CS group compared with those in the normal group and the SPS group（P0.05）.However,the times of head-dipping and rearing showed no significant differences among the three groups.（4） The Morris water maze test showed that the escape latency had no significant difference among three groups on the first and the second day,but the escape latency in the CS group（35.67±5.13,25.09±3.55） was significantly longer than that of the normal group and SPS group on the third and the fourth day（P0.01,P0.05）.In the spatial probe test,the times of entering target quadrant and crossing hidden platform significantly decreased in the CS group（5.80±2.34,3.10±1.79） compared with those in the normal group and SPS group（P0.01,P0.05）.Conclusions The rat behavior test confirms that compound stress is a simple and effective method for the establishment of PTSD model in rats.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2012年第10期928-932,共5页
Journal of Third Military Medical University
基金
全军医药卫生科研基金(08G096)~~
关键词
PTSD
连续单一应激
复合应激
旷场实验
高架十字迷宫
post-traumatic stress disorder
Single-prolonged stress
compound stress
openfield test
elevated plus maze
