摘要
目的鸦胆子油乳具有抗肿瘤、提高机体免疫力和诱导细胞凋亡等作用。本研究以小鼠肺癌恶病质模型为研究对象,评价并探讨鸦胆子油乳的治疗效果与作用机制。方法建立Lewis肺癌小鼠恶病质动物模型45只,随机分成荷瘤空白对照组、鸦胆子油乳治疗组和消炎痛治疗组3组,每组各15例。恶病质状态出现后,分别给予鸦胆子油乳、消炎痛及等量生理盐水治疗。观察小鼠体质量、摄食量及饮水量,测定小鼠血清中恶病质相关细胞因子肿瘤坏死因子α(tumor necrosis factor-alpha,TNF-α)和白细胞介素6(interleukin6,IL-6)水平。结果治疗后,鸦胆子油乳治疗组小鼠的摄食量为(2.41±0.17)g,明显高于荷瘤空白对照组的(2.09±0.09)g,t=2.353,P=0.015;鸦胆子油乳治疗组小鼠饮水量为(3.23±0.26)mL,明显高于荷瘤空白对照组的(2.77±0.17)mL,t=2.617,P=0.012;鸦胆子油乳治疗组小鼠的体质量为(20.08±1.37)g,明显高于荷瘤空白对照组(18.71±1.64)g和消炎痛治疗组(18.88±2.23)g,t值分别为2.363和2.232,P值分别为0.021和0.025。经鸦胆子油乳治疗后恶病质荷瘤小鼠血清中IL-6为(83.99±48.72)pg/mL,TNF-α为(8.50±6.02)fmol/mL,水平明显低于荷瘤空白对照组IL-6(200.94±99.57)pg/mL和TNF-α(26.79±13.79)fmol/mL,t值分别为6.184和5.959,P值分别为0.006和0.008。结论鸦胆子油乳可以明显改善癌症恶病质相关的体质量下降、摄食量及饮水量减少等症状,其机制可能与下调恶病质相关细胞因子IL-6和TNF-α水平有关。
OBJECTIVE Currently no specificity in the treatment of cancer cachexia,thisstudypreliminary study of Java brucea fruit oil emulsion＇s mechanism of anti-cancer cachexia by observing of cancer cachexia-related physiological Indicators and serum cytokine TNF-a,IL-6 level. METHODS After establishing Lewis lung cancer in miceanimal model of cancer cachexia 45 cases,the mice randomly divided into three groups,including the group of no treatment with tumor （GNT）, the group of Java brucea fruit oil emulsion ~s treatment（GYT）, the group of indomethacin s treatmen（GIT）, each 15 cases. When cachexia state appears,all mice were respectively given the treatment of Java brucea fruit oil emulsion,in- domethacin and NS. Mice are observed body weight,food intake,water intake, then determine the serum cytokine TNF-a, IL-6 level. RESULTS Aftertreatmet,Compared with GNT,not only food intake and drinking water of GYT＇s mice was significantly increased （t= 2. 353,2. 617 ; P 〈 0. 015,0. 012） ; The body weight of the GYT＇ s mice increased significantly higher than the GNT＇s and GIT＇s mice （t=2. 363,2. 232;P〈0. 021,0. 025）. The serum IL-6,TNF-a levels of GYT＇s mice were significantly lower than GIT＇s （t=6. 184,5. 959;P〈0. 006,0. 008）. CONCLUSIONS Java brucea fruit oil e- mulsion can significantly improve the decline in body weight and the reduction of food intake and water intake caused by the cancer eachexia, the mechanic may related with Java brucea fruit oil emulsion can reduce cancer cachexia related serum cytokines IL-6 ,TNF-a levels.
出处
《中华肿瘤防治杂志》
CAS
北大核心
2017年第18期1280-1283,共4页
Chinese Journal of Cancer Prevention and Treatment
