摘要
目的:分离、鉴定鸦胆子内生真菌,并考察其抑菌活性。方法:采用组织分离法对鸦胆子内生真菌进行分离,并根据形态特征进行鉴定。采用对峙法和管碟法分别测定对7种植物病原真菌和3种细菌的抑制作用。结果:从鸦胆子的根、茎、叶和果实共分离到83株内生真菌,其中,茎分离纯化得到34株内生真菌;叶分离纯化得到32株内生真菌;根分离纯化得到15株内生真菌;果实分离纯化得到2株内生真菌。已鉴定的73株内生真菌分属于5目6科12属,其中14株内生真菌对至少1种植物病原真菌有拮抗活性;9株内生真菌的代谢产物对1种以上细菌有抑制作用,尤其是拟茎点霉属菌株YJ-17对指示菌的抑菌活性较强。结论:鸦胆子内生真菌种类丰富,且具有抑菌活性,值得在病害防治方面作进一步深入研究。
Objective： To isolate and identify endophytic fungi from Brucea javanica,and to detect the antimicrobial activity of these strains.Methods： Endophytic fungi were isolated by tissue inoculation culture and identified by conventional morphological characteristic method.Seven kinds of pathogenic fungi and three kinds of bacteria were used as targeting microbes to test microbial inhibition activities by agar plate antagonistic action and modified agar gel diffusion methods,respectively.Results： A total of 83 endophytic fungi strains were isolated from the root,stem,leaf and fruit of Brucea javanica.34 strains were obtained from the stem,32 strains were obtained from the leaf,15 strains were isolated from the root and 2 strains came from the fruit.These 73 strains which had been identified attribute to5 orders,6 families and 12 genera.For the isolated strains,14 strains had antifungal activities against at least one pathogenic fungi,9strains showed antibacterial activities against one or more bacteria.Especially,the strain YJ-17 which belonged to Phomopsis genus showed the best inhibitory effect on the targeting microbes.Conclusion： The endophytic fungi from Brucea javanica show diversity and microbial inhibition activity,and are worthy for further study on plant disease controlling.
出处
《中药材》
CAS
CSCD
北大核心
2014年第4期564-568,共5页
Journal of Chinese Medicinal Materials
基金
广西自然科学基金创新研究团队项目(2011GXNSFF018006)
广西自然科学基金(2012GXNSFAA053119)
广西科技合作与交流计划(1298015-6)
桂林市科技攻关与新产品(20100305)
关键词
药用植物
鸦胆子
内生真菌
分离
抑菌活性
Medicinal plant Brucea javanica（L.） Merr. Endophytic fungi Isolation Microbial inhibition activity
