摘要
[目的]探讨养正散结汤抑制裸鼠胃癌移植瘤生长的作用机制。[方法]建立人胃癌裸鼠皮下移植瘤模型,随机分为阴性对照组、阳性对照组(卡莫氟组)、养正散结汤高、中、低剂量组。连续治疗3周,实验结束后处死荷瘤鼠,剥取肿瘤,称重,计算抑瘤率;免疫组化法检测肿瘤组织Ki-67蛋白表达,计算肿瘤细胞增殖指数;采用原位杂交技术检测肿瘤组织let-7a表达,免疫组化法检测c-Myc蛋白表达。[结果]养正散结汤高、中、低剂量组肿瘤重量明显小于阴性对照组,增殖指数显著低于阴性对照组;各组肿瘤组织let-7a表达显著高于阴性对照组,c-Myc蛋白表达显著低于阴性对照组。[结论]养正散结汤具有显著的抑制胃癌生长的作用,其机制可能是上调抑癌性miRNA let-7a、抑制其靶基因c-Myc蛋白表达,从而抑制胃癌细胞增殖。
[Objective]To explore the molecular mechanisms underlying the anti-cancer effect of Yangzheng Sanjie decoction（YZSJD）in vivo.[Methods]Nude mice bearing human gastric cancer MKN-45 cells were randomly divided into five groups：control group,Carmofur group,and high/middle/low doses of YZSJD groups.All mice were sacrificed after three weeks of treatment.Tumor weights were measured and tumor inhibition rates were calculated.Let-7aexpression was examined by in situ hybridization.Ki-67 tumor cell proliferation index and c-Myc protein expression were determined by immunohistochemistry.[Results]YZSJD significantly inhibited tumor growth of subcutaneous MKN-45 gastric cancer xenograft in nude mice.Ki-67 proliferation indexes were much lower in YZSJD groups than those in control group.YZSJD treatment resulted in significant up-regulation of let-7alevels,accompanied by a marked decrease in c-Myc expression.[Conclusion]YZSJD suppresses the growth of gastric cancer in vivo by regulating let-7a/c-Myc expression.
出处
《中国中西医结合消化杂志》
CAS
2017年第10期738-741,746,共5页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
国家自然科学基金资助项目(No:81273735
No:81373563)
广东省省级科技计划项目(No:2013B021800169)
广东省中医院中医药科学技术研究专项课题(No:YN2014ZH05)
