摘要
[目的]研究铁树种子提取物的抑菌活性。[方法]采用纸片扩散法、牛津杯法、琼脂稀释法和荧光染色法,研究提取物抑菌活性。[结果]提取物具有光谱抗菌活性,对禾谷镰刀菌的IC_(50)为1.65 mg/mL,对其分生孢子的IC_(50)为2.93 mg/mL,改变禾谷镰刀菌菌丝尖端几丁质的量和线粒体内膜负电性。[结论]提取物具有较好的抑菌活性。
[ Objective] The aim was to study antibacterial activity of extract of Cycas siamensis seed. [ Method] The antibacterial activity of ex-tract of Cycas siamensis seed was studied by filter paper method, agar dilution method and fluorescent staining method. [ Result ] The extract had a broad-spectrum antibacterial activity, and the IC50 of Fusarium graminearum and spores were 1. 65 and 2. 93 mg/mL. The extracts changed hypha chitin and mitochondrial membrane potential. [ Conclusion]The extract has stronger antibacterial activity.
出处
《安徽农业科学》
CAS
2017年第31期12-14,17,共4页
Journal of Anhui Agricultural Sciences
关键词
苏铁
种子提取物
病原菌
抑菌活性
Cycas siamensis
Seed extracts
Pathogenic bacteria
Antibacterial activity