摘要
目的探讨重症急性胰腺炎(SAP)大鼠脏器功能障碍及病程演进规律。方法本研究选取雄性远交系大鼠80只,模型组和对照组各40只。模型组采用经胰管逆行注射3.5%牛磺胆酸钠诱导SAP,对照组予胰管内注射生理盐水。分别于模型诱导后3、6、12、24 h(每一时间点各10只大鼠)处死,测定大鼠血淀粉酶、肌酐、尿素氮;光镜观察胰腺组织和肾组织病理学改变。结果模型组大鼠血淀粉酶活性及12 h后肌酐、尿素氮浓度较对照组明显升高(P<0.05)。病理组织学观察可见造模完成后随时间进程胰腺和肾损伤呈加重表现。结论通过逆行胰管注入3.5%牛磺胆酸钠法成功建立了大鼠SAP相关性肾损伤动物模型,为SAP相关性肾损伤的发病机制及药物干预研究提供了一种较理想的动物模型。
Objective To investigate the evolution of organ dysfunction and disease course in rats with severe acute pancreatitis（ SAP）. Methods All 80 rats were randomly divided into SAP group and normal control group. Each group included 40 rats. SAP was induced by retrograde pancreatic duct injection of 3. 5% sodium taurocholate in SAP group. Pancreatic duct was injected normal saline in the normal control group. After the model had been induced,10 rats in each group were killed at 3 h,6 h,12 h and 24 h. Every rat＇s serum AMY,Cr and BUN was detected. The difference of their pancreatic tissue and renal tissue pathology through light microscope was observed. Results Compared with normal control group at 3 h,6 h,12 h and 24 h,the levels of serum AMY in the SAP group significantly increased. When 12 h later,the levels of serum Cr and BUN increased significantly,compared with normal control group（ P〈0. 05）.Pathologic histology observation showed that the time course of the pancreas and kidney injury was aggravated after the completion of the model. Conclusions The animal model of SAP related renal injury in rats was established successfully by retrograde pancreatic duct injection of 3. 5% sodium taurocholate,which was an ideal animal model for the study of the pathogenesis and drug intervention of SAP related renal injury.
出处
《中华胃肠内镜电子杂志》
2017年第2期70-74,共5页
Chinese Journal of Gastrointestinal Endoscopy(Electronic Edition)
基金
镇江市2013年度科技支撑计划(社会发展)指导性项目(FZ2013006)
