摘要
目的考察海藻多糖(SP)对异烟肼(INH)与利福平(RIF)合用所致氧化型肝损伤的保护作用以及对肝脏转运体Na+-牛磺胆酸共转运多肽(Ntcp)的调控作用。方法 Wistar雄性大鼠24只,随机分为正常组、INH-RIF组和SP组,每组8只。INH-RIF组:灌胃INH和RIF各100 mg/(kg·d);SP组:灌胃SP 720 mg/(kg·d),3 h后灌胃INH和RIF各100 mg/(kg·d)。在给药21 d后,处死大鼠,通过血清生化指标、肝脏病理、氧化指标及Ntcp蛋白表达对各组大鼠进行评价对比。结果与INH-RIF组相比,SP组明显降低血清天冬氨酸氨基转移酶、丙氨酸氨基转移酶和总胆汁酸(P<0.05或P<0.01),缓解肝脏的病变;能降低丙二醛含量,增加肝脏谷胱甘肽含量,对Ntcp的表达具有明显的上调作用(P<0.05或P<0.01)。结论 SP能明显改善INH与RIF诱导的肝脏氧化损伤,且能增加肝脏转运体Ntcp的表达,缓解肝脏病变。
Objective To preliminarily study the effects of seaweed polysaccharide on isoniazid （INH） rifam- picin （RIF） induced liver injury and the expression of Na＋-taurocholate cotransporting polypeptide （Ntcp）. Methods Rats were randomly divided into 3 groups, namely a normal group, INH＋RIF group and seaweed polysaccharide group, with eight in each. The INH-RIF group was administered the INH （100 mg/（kg d）） and RIF （100 mg/（kg, d）） via oral administration for 21 days. The seaweed polysaccharide group was orally admin- istered seaweed polysaccharide （720 mg/（kg, d）） 3 hours before 1NH and RIF administration for 21 days. Then the rats were evaluated through comparing their biochemical indicators of blood serum, hepatic pathol- ogy, liver GSH and MDA and the expression of Ntcp. Results Compared with the INH and RIF group, the serum AST, ALT and TBA were significantly decreased, liver GSH was significantly increased and liver MDA was decreased, and the expression of Ntcp was significantly increased in seaweed polysaccharide group. Conclusion Seaweed polysaccharide could markedly relieve the INH and RIF induced liver injury of rats and up-regulate the expression of Ntcp.
出处
《兰州大学学报（医学版）》
CAS
2017年第4期23-27,共5页
Journal of Lanzhou University（Medical Sciences）
基金
国家自然科学基金项目(81041086)
关键词
海藻多糖
异烟肼
利福平
氧化损伤
Na+-牛磺胆酸共转运多肽
seaweed polysaccharide
isoniazid
rifampicin
oxidative damage
Na＋-taurocholate cotransport- ing polypeptide
