摘要
建立了一种高效液相色谱法等度分析克拉霉素及其4种相关物J、M、N和K的检测方法,并对其进行了方法学验证.采用Purospher STAR LP RP-18e型色谱柱,用0.067mol/L KH2PO4溶液(pH4.0)/乙腈=60/40作为流动相,流速为1.2mL/min,柱温为35℃,在205nm进行检测,对克拉霉素及其4种相关物进行等度洗脱,获得了较好的分离度,均大于1.2.该方法在各相关物20~240μg/mL的浓度范围内线性良好(R>0.999).平均回收率为87.80%~121.17%.本方法简便、高效,能够同时检测克拉霉素中的J、M、N和K几种相关物.
An effective method was established and verified for the detection of clarithromycin and its relative substances J,M,N and K by HPLC.Clarithromycin relative substances sample was separated by isocratic elution with a Purospher STAR LP RP-18e C18 column and with the mobile phase 0.067mol/L KH_2PO_4 solution（pH 4.0）/acetonitrile= 60/40,the flow rate of 1.2mL/min,the column temperature 35℃ and detection wavelength 205 nm,and the separation degrees were greater than 1.2.Analysis results show that,the method can provide good linearity（R0.999）for all relative substances at the concentration range of 20~240μg/mL,the average recovery rates keep at 87.80%~121.17%,its operation is simple and efficient,and can be able to simultaneously detect J,M,N and K in clarithromycin.
出处
《北京理工大学学报》
EI
CAS
CSCD
北大核心
2017年第9期986-990,共5页
Transactions of Beijing Institute of Technology
关键词
克拉霉素
相关物
HPLC
检测
established
detection
clarithromycin
relative substances
HPLC
Clarithromycin relative
substances sample
isocratic elution
