摘要
采用辐照法制备交联聚丙烯酰胺毛细管电泳涂层柱。毛细管先经双功能团试剂γ－ 甲基丙烯基氧丙基三甲氧基硅烷预处理，然后柱内充入丙烯酰胺溶液进行辐照聚合，得到线 性涂层柱；再经动态涂覆交联剂甲叉双丙烯酰胺，二次辐照交联，采用随机试验设计方法，对 辐照剂量率、辐照时间和交联剂浓度几个因素进行了优化。在ｐＨ ４、３３３ Ｖ／ｃｍ场强下，碱性 和酸性蛋白的平均柱效可达９．５ ｘ１０５和１．６９ｘ１０５理论塔板／米。对不同场强下的柱效、迁移 时间和分高度进行了研究。
A method for the preparation of cross-linked polyacrylamide coated capillary by radiation- induced polymerization is presented. The capillary was pretreated with γ- methacrylo xypropyltrimethoxysilane. Three percents of acylamide solution were flushed in the capillary and γ-60 Co was used to initiate polymerization. The capillary was then coated dynamically with methylene bisacrylamide and subjected for radiation-induced crosslinking. Random design method was exploited in the optimization of radiation dose, time and concentration of methylene bisacrylamide. The preparation method is simple, fast , reproducible, and suitable for batchwise processing. The polymerization reaction was carried out at room temperature and without catalyst remains. Theoretical plates reached for basic and acidic proteins at a field strength of 333 V/cm and pH 4 are 9. 8× 105 and 1. 69 × 105 , respectively. Acidic proteins were well separated with basic buffers. The theoretical plates and resolution were found to be inversely proportional to the field.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1998年第11期1303-1308,共6页
Chinese Journal of Analytical Chemistry
关键词
毛细管电泳
涂层柱
聚丙烯酰胺
蛋白质
分离
Capillary electrophoresis, coated capillary, polyacrylamide, irradiation induced polymerization
