摘要
目的:检测中性粒细胞亚型哮喘(NA)患者痰上清中辅助性T细胞17(Th17)特异性转录因子维甲酸相关核孤儿受体γt(RORγt)、B细胞转录激活因子(BATF)及外周血中Th17细胞相关细胞因子白细胞介素8(IL-8)、白细胞介素17(IL-17)和白细胞介素22(IL-22)表达水平,探讨其在NA发病中的作用。方法:以56例支气管哮喘患者为研究对象,4.5%生理盐水雾化诱导痰,经细胞MGG染色进行哮喘的炎症亚型分型,分为嗜酸性粒细胞亚型哮喘(EA)组(26例)和NA组(30例),以常规体检者为健康对照组(NC组,28人)。实时荧光定量PCR(RT-PCR)法检测痰上清中BATF和RORγt mRNA表达水平。空腹抽取各组研究对象肘静脉血10mL,Ficoll密度梯度离心后分离外周血单个核细胞(PBMC),ELISA法测定血清中IL-8、IL-17和IL-22蛋白表达水平,流式细胞术检测PBMC中Th17细胞(CD4+IL-17+细胞)的百分率。结果:与NC组和EA组比较,NA组患者血清中IL-8、IL-17和IL-22表达水平均明显升高(P<0.05);与NC组比较,EA组患者血清中IL-8、IL-17和IL-22表达水平差异无统计学意义(P>0.05)。与NC组和EA组比较,NA组患者痰上清中BATF和RORγt mRNA表达水平明显升高(P<0.01)。与NC组和EA组比较,NA组患者PBMC中Th17细胞百分率均明显升高(P<0.01);与NC组比较,EA组患者PBMC中Th17细胞百分率差异无统计学意义(P>0.05)。结论:IL-17转录因子RORγt和BATF参与了NA患者气道炎症发生过程,且Th17细胞及其相关细胞因子IL-17和IL-22的异常表达与NA的全身反应有关联。
Objective： To detect the expression levels of retinoic acid-related orphan receptor γt （RORγt）,B cell activating transcription factor（BATF） in sputum of the patients with neutrophilic asthma（NA）,Th17 cells-related cytokines such as interleukin-8（IL-8）,IL-17, and IL-22 in peripheral blood,and to investigate their effects in the pathogenesis of NA.Methods： Fifty-six patients with asthma were selected as subjects and the sputum was induced with 45% normal saline.According to the results of sputum smear by MGG staining,they were divided into eosinophilic asthma group （EA,n=26） and NA group （n=30）.28 cases of healthy volunteers were selected as control group. The expression levels of IL-8,IL-17, and IL-22 in serum were detected by ELISA and the expressions of BATF and RORγt mRNA in sputum were determined by RT-PCR. And T-lymphocytes were collected from the peripheral blood mononuclear cells （PBMC）,and then the percentages of Th17 cells were determined by flow cytometry.Results： Compared with NC and EA groups,the levels of IL-8,IL-17, and IL-22 in serum of the patients in NA group were significantly increased （P〈0.05）,but there were no significant differences between EA and NC groups（P〉0.05）.Compared with NC and EA groups,the expression levels of BATF and RORγt mRNA were significantly increased （P〈0.01）; compared with NC and EA groups, the percentage of Th17 cells in PBMC of the pathents in NA group was significantly increased （P〈0.01）,but there was no significant difference between EA and NC groups（P〉0.05）.Conclusion： RORγt and BATF involve in the onset and development of airway inflammation in the patients with NA,and Th17 cells as well as Th17-related cytokines such as IL-17 and IL-22 may be closely associated with systematic resposes of NA.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2017年第1期106-110,共5页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科技发展计划-科技创新人才培育计划资助课题(20150519015JH)
吉林大学大学生创新创业训练计划国家级项目资助课题(2015791156)
吉林大学白求恩B计划资助课题(2012218)
