摘要
目的早期食管癌临床症状常不明显,肿瘤标志物的深入研究对食管癌早期诊断极为重要。文中通过研究体外特定细胞株[Eca-109及人食管正常上皮细胞(HEEC)]中Rock2和Wnt11蛋白表达量,分析各蛋白的表达与食管癌的关系。方法培养Eca-109和HEEC细胞株,RT-q PCR和Western blot技术检测Rock2和Wnt11在细胞株中的表达水平。结果RT-q PCR检测结果显示,与HEEC相比,Eca-109中Rock2 mRNA、Wnt11 mRNA表达水平明显升高[(1.000±0.000)vs(4.955±0.539),(1.000±0.000)vs(2.925±0.230),P<0.01]。Western blot检测显示Rock2和Wnt11蛋白在Eca-109中的表达较HEEC均升高[(955.000±21.628)vs(778.844±102.193),(2 175.316±145.623)vs(1 312.233±50.734),P<0.05]。结论 Rock2和Wnt11表达增高可能是食管鳞状细胞癌转移的标志。
Objective Early clinical symptoms of esophageal cancer are often not obvious,so a deeper insight into tumor markers is very important for the early diagnosis of esophageal cancer. This study was to investigate the expressions of Rock2 and Wnt11 in the specific esophageal cancer cell lines Eca-109 and HEEC and the relationship of the signal transduction pathway of proteins with the development of esophageal cancer. Methods Esophageal cancer cell lines Eca-109 and HEEC were cultured and the expression levels of Rock2 and Wnt11 in the cell lines were determined by real-time quantitative PCR and Western blot. Results The mRNA expressions of Rock2 and Wnt11 were significantly increased in the Eca-109 as compared with those in the HEEC cell line（ 4. 955 ±0.539 vs 1.000±0.000,P〈0.01; 2.925±0.230 vs 1.000± 0.000,P 〈0.01）,and so were the protein expressions of Rock2 and Wnt11（ 955.000±21.628 vs 778.844±102.193,P〈0.05; 2175.316±145.623 vs 1312.233±50.734,P〈0.05）. Conclusion The up-regulated expressions of Rock2 and Wint11 may be the markers of the metastasis of esophageal squamous cancer.
出处
《医学研究生学报》
CAS
北大核心
2017年第1期10-13,共4页
Journal of Medical Postgraduates
基金
国家自然科学基金(81260308)
