摘要
目的泛素-蛋白酶系统(UPS)在食管癌的发生、发展中具有重要作用。泛素分子(UB)作为UPS的执行者,在食管癌发病过程中的表达情况及其临床诊断价值鲜有报道。文中探讨血清UB在食管鳞癌(ESCC)中的表达及其诊断价值。方法纳入2018年2月至2019年5月南通大学附属江阴医院收治的88例ESCC患者(ESCC组)及40例进行食管癌筛查无异常者(正常对照组)。采用ELISA法测定血清UB,化学发光法测定血清鳞状细胞癌抗原(SCC)和癌胚抗原(CEA)。采用ROC曲线分析各指标诊断价值,并分析血清UB与临床病理特征的关系。结果与正常对照组相比,ESCC组患者血清UB水平显著升高[(41.96±3.273)ng/mL vs(80.86±7.993)ng/mL,P<0.05]。在有无淋巴转移及肿瘤不同分期患者中,血清UB表达差异有统计学意义(P<0.05)。UB、SCC、CEA以及三者联合诊断ESCC的灵敏度分别为65.9%、52.3%、51.1%、76.1%;ROC曲线下面积分别为:0.690、0.667、0.635、0.795。结论血清UB在ESCC中高表达,且与肿瘤进展密切相关。联合血清UB与SCC、CEA可提高诊断ESCC的灵敏度,并可作为有效的血清学筛查指标。
Objective Ubiquitin-proteasome system(UPS)plays a central role in the development of esophageal cancer.However,As the executor of UPS,the expression and clinical significance of detection of serum ubiquitin(UB)in Esophageal Squa-mous Cell Carcinoma(ESCC)patients have not been fully elucidated.The aim of this study was to investigate the expression and diag-nostic value of serum ubiquitin(UB)in ESCC.Methods A total of eighty-eight ESCC patients and forty healthy controls from Feb-ruary 2018 to May 2019 at the Affiliated Jiangyin Hospital of Nantong University were enrolled.Serum UB was measured by ELISA,and serum squamous cell carcinoma antigen(SCC)and carcinoembryonic antigen(CEA)were determined by chemiluminescence method.The ROC curve was used to analyze the diagnostic value of each indicator.Besides,the correlation between serum UB and clinicopathological features of ESCC were analyzed.Results The level of serum UB in ESCC group was significantly higher than that in the control group[(41.96±3.273)ng/ml vs(80.86±7.993)ng/ml,P<0.05].The level of serum UB in ESCC patients was related to lymphat-ic metastasis and tumor stage(P<0.05).The sensitivity of UB,SCC,CEA and the three combined diagnosis of ESCC were 65.9%,52.3%,51.1%,and 76.1%,respectively.The AUC under the ROC curve were 0.690,0.677,0.635,and 0.795,respectively.Conclusion Serum UB is highly expressed in ESCC and is closely related to tumorprogression.Combined with SCC and CEA,UB can improve the sensitivity of diagnosis of ESCC and can be used as an effective sero-logical screening biomarker.
作者
徐依人
刘鹏飞
贾慧敏
高昳
王芳军
沈卫东
李腾飞
XU Yi-ren;LIU Peng-fei;JIA Hui-min;GAO Yi;WANG Fang-jun;SHEN Wei-dong;LI Teng-fei(Department of Gastroenterology,the Affiliated Jiangyin Hospital of Nantong University Jiangyin 214400,Jiangsu,China;School of Radiation Medicine and Protection,Medical College of Soochow University,Soochow 215000,Jiangsu,China)
出处
《医学研究生学报》
CAS
北大核心
2019年第12期1276-1279,共4页
Journal of Medical Postgraduates
基金
国家自然科学基金(31670857,31700737)
江苏省自然科学基金(BK20161152)
关键词
血清泛素
食管鳞癌
鳞状细胞癌抗原
癌胚抗原
ubiquitin
esophageal squamous cell carcinoma
serum squamous cell carcinoma antigen
carcinoembryonic antigen
