摘要
目的探讨熊果酸对人肝癌细胞Hep G2及小鼠肝细胞AML^(-1)2胆固醇代谢的影响及其相关机制。方法0,10,20,40μmol·L^(-1)熊果酸处理Hep G2及AML^(-1)2细胞24 h,应用反转录-聚合酶链反应(RT-PCR)、Western blotting检测细胞内胆固醇代谢关键基因胆固醇7A-羟化酶(CYP7A1)mRNA和蛋白表达,应用酶法检测细胞内胆固醇浓度。结果与0μmol·L^(-1)熊果酸组比较,20和40μmol·L^(-1)熊果酸组Hep G2及AML^(-1)2细胞内CYP7A1 mRNA及蛋白表达水平均显著增加(均P<0.05),相应的细胞内胆固醇浓度也随之下降(均P<0.05)。结论一定浓度的熊果酸能够降低肝细胞内胆固醇浓度,CYP7A1可能参与了调节过程。
Objective To explore the effects and mechanism of ursolic acid（ UA） on cholesterol metabolism in human hepatocellular carcinoma HepG2 and mouse hepatocyte AML-12. Methods HepG2 and AML-12 cells were treated with different concentrations of UA（ 0,10,20,40 μmol·L^-1） for 24 h,then the mRNA and protein expression of cholesterol 7alphahydroxylase（ CYP7A1） and intracellular cholesterol level was detected by RT-PCR、Western blotting and enzymatic method,respectively. Results Compared with 0 μmol·L^-1UA,20 μmol·L^-1and 40 μmol·L^-1UA significantly increased the expressions of CYP7A1 mRNA and protein（ P〈0.05）,and decreased intracellular cholesterol level in HepG2 and AML-12 cells（ P〈0.05）. Conclusion A certain concentration of UA can reduce the level of cholesterol in HepG2 and AML-12 cells.CYP7A1 may be involved in the regulation process.
出处
《医药导报》
CAS
2017年第1期9-12,共4页
Herald of Medicine
基金
国家自然科学基金资助项目(81202191)
黑龙江省普通高等学校营养与食品卫生学重点实验室开放课题(YYKFKT1209)
关键词
熊果酸
胆固醇代谢
胆固醇7A-羟化酶
Ursolic acid
Cholesterol metabolism
Cholesterol 7alpha-hydroxylase
