摘要
霉菌是酿酒大曲菌群的重要组成部分,其产酶能力影响大曲质量。对宋河中高温大曲和高温大曲中的霉菌进行分离纯化,采用形态学和18Sr DNA基因分析对主要霉菌进行鉴定,并采用透明圈法初筛主要产酶菌,半固态发酵测酶活法研究几株初筛霉菌的产酶功能。结果表明:共得到具有明显特征的10株霉菌,9株分别归属于4个属即为青霉属(Penicillium)、曲霉属(Aspergillus)、毛霉属(Mucor)和链格孢属(Alernaria),1株未确定;初筛得到7株均能产生淀粉酶、蛋白酶、纤维素酶和酯化酶的功能霉菌,其中菌株MM9产α-淀粉酶和蛋白酶的活力较高,其酶活分别为32.45U/m L和587.50U/m L,菌株Mh2产纤维素酶活力最高达11.87U/m L;菌株MM10产酯化酶活力最高为16.03U/m L。
The function of enzyme production by mould which are an important part of fungal community in Daqu affects the the quality of Daqu. Important mould isolated from Songhe mid-temperature and high-temperature Daqu were identified with the methods of observing physical characterization and 18 Sr DNA gene analysis, Observing transparent circle in plates and determining enzyme activity of the semi-solid state fermentation broth by mould were carried out to preliminary select important enzyme-producting mould and research its function of enzyme production.The results were as follows： 10 mould strains that had obvious properties were isolated and purified, nine of which were classified into 4 genera morphologically： Penicillium, Aspergillus, Mucor, Alernaria, one of which was unidentified. 7 strains all could product α-amylase, protease, cellulase; esterifying enzyme were obtained through the transparent circle screen. By semi-solid state fermenting, strain MM9 had the higher activity of α-amylase and protease which reached 32.45U/m L and 587.5U/m L, respectively. The highes activity of celluase enzyme reached 11.87U/m L produced by mould was strain Mh2. The strain could produce the highest esterifying enzyme reached 16.03U/m L was MM10 strains.
作者
李绍亮
李学思
侯小歌
王俊英
LI Shaoliang LI Xues HOU Xiaoge WANG Junying(Henan Songhe Distillery Co. Ltd., Luyi 477265, China College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou,China)
出处
《酿酒》
CAS
2016年第6期24-29,共6页
Liquor Making
