摘要
[目的]对一种商业化的低血清培养基适应BHK21细胞进行传代培养，并分析该细胞染色体的变异稳定性，以判定其质量。[方法]采用直接法制备4个代次BHK21细胞的染色体标本，用常规Giemsa染色，在1000倍光学显微镜下计数中期分裂相染色体数目，并进行核型分析。[结果]BHK21为亚二倍体或亚四倍体细胞系，亚二倍体总数为42，亚四倍体细胞总数为72～74，该细胞系染色体的畸变率在各代次所占比例为0～2％，均较低。[结论]该细胞系的遗传学特性相对稳定，各代次间无本质差异，可候选为制苗用细胞。
[Objective] To analyze a commercialization low serum medium, which was suitable for subculture of BHK21 cells, to analyze the var-iation stability of the cell chromosome to determine its quality.[Method] Direct method was used to prepare chromosome specimens of 4 four generations BHK21 cells.Conventional Giemsa staining was adopted.The number of metaphase mitotic chromosomes was counted by 1 000 times of optical microscope, so as to find the rate of chromosome mutation, and to carry out karyotype analysis.[Result] BHK21 was a sub diploid or tetraploid cell line.The hypodiploid mode was 42, hypotetraploid cell mode was 72-74 .The chromosome aberration rate of the cell line was 0-2%., which was lower than that of the generation.[Conclusion] The genetic characteristics of the cell line are relatively stable, there is no es-sential difference between the generations, and it can be used as a candidate for the production of cells.
出处
《安徽农业科学》
CAS
2016年第23期97-98,190,共3页
Journal of Anhui Agricultural Sciences
基金
国家高新技术研究发展计划项目("863"计划2011AA10A211)
