摘要
目的应用染色体核型分析技术对白血病患者进行染色体畸变检测,分析它们对白血病分型诊断和预后判断的临床意义。方法疑似白血病患者118例,染色体核型分析采用骨髓细胞短期培养法、G显带。结果疑似白血病患者染色体畸变率为61.86,其中和法美英合作小组(FAB)亚型相关的特异染色体重排占染色体畸变的72.60。按FAB分类,M1、M2a、M2b、M3、M4、M5、慢性髓细胞性白血病(CML)、L2和骨髓增生异常综合征的染色体畸变率分别是50.00、56.00、83.33、70.59、40.00、66.67、92.59、44.44、20.00。t(8;21)仅出现在M2,t(15;17)仅出现在M3,t(9;22)出现在CML和L2。其中,CML的t(9;22)的阳性率为92.60,L2的t(9;22)的阳性率为18.18。按世界卫生组织的分类法,染色体核型预后良好组、预后不良组和中间组的白血病缓解率分别是88.89、33.33和64.71,差异有统计学意义(P<0.01)。结论染色体核型分析对白血病的分型诊断和预后判断具有重要参考价值。
Objective To detect chromosomal aberrations in patients with leukemias by karyotype analysis and analyze the clinical significance of related results for the typing diagnosis and prognosis of leukemias. Methods Chromosomes from 118 cases of suspected patients with leukemias were prepared after 24 hours culture of bone marrow and G-banding were used to analyze karyotypes. Results Chromosomal aberrations were detected in 61. 86% of suspected patients. 72.60% of detected chromosomal abnormalities were specific chromosome rearrangement related with FAB types. According to FAB types, chromosome abbreviation frequency of M1,MZa,MZb,M3,M4,M5,CML,LZ and MDS was 50.00%,56.00%,83.3%,70.59%,40%,66.7%,92.6%,44.4% and 20% ,respectively. t（8：21） was only detected in patients with M2 and t（15;17） in patients with M3. t（9：22） was detected in patients with CML or L2. Abbreviation frequency of t（9：22） was 92.60% in CML and 18.18% in L2. According to WHO types, remission rates of patients with good--prognosis, poor--prognosis and moderate prognosis of karyotype were 88.89%, 33.33% and 64.71% respectively and there was significant difference among them（P〈0.01）. Conclusion Karyotype analysis is helpful for the diagnosis and prognosis of leukemias.
出处
《国际检验医学杂志》
CAS
2010年第12期1359-1360,1363,共3页
International Journal of Laboratory Medicine
关键词
染色体
白血病
诊断
预后
chromosome
leukemia
diagnosis
prognosis
