摘要
以烟草赤星病抗源材料净叶黄总ＤＮＡ为供体，感病材料ＮＣ８９为受体，通过涂抹柱头法和注射子房法进行了外源ＤＮＡ导入处理。在人工接种及自然发病条件下，对处理后代进行了赤星病抗性鉴定。结果约２％～４％的处理材料抗性较ＮＣ８９有所提高，个别植株已接近净叶黄。从中选取３株抗赤星病性状及农艺性状均表现较好的单株，自交收种后于次年在大田条件下对其后代（Ｄ＿２代）的抗性性状进行遗传稳定性分析，结果其中１个株系（ＴＮ－Ａ＿１）的抗赤星病性状可以稳定遗传。
The total DNA of donor tobacco cultivar Jingyiehuang,which is resistant to A. al-ternata,was isolated in fragments of 10￣6～10￣7 daltons and then introduced into the recipienttobacco cuItivar NC89 sensitive to A. alteruata by ovule-injecting and stigma- smirching,re- spectively.The resistance of the first generation was identified under mechanical inocula-tion and natural infection conditions,and about 2%～4%of them were found to exhibithigher resistance than the control NC89 plants.The results indicated that the resistantgene of the donor plant could be possibly integrated into or recombined with the recipientgenome by the techniques described above.
出处
《河南农业大学学报》
CAS
CSCD
1995年第1期71-75,共5页
Journal of Henan Agricultural University
