摘要
目的比较甜牛大力和苦牛大力对免疫抑制小鼠的免疫调节作用。方法昆明种(KM)小鼠随机分为正常对照组、环磷酰胺(CTX)组(模型组)、左旋咪唑(LMS)组(阳性对照组)与高、中、低剂量(20、10、5g/kg)的甜牛大力(Radix Millettia Speciosa,RM Speciosa)组及苦牛大力(Radix Millettia Championi,RM Championi)组,连续灌胃给药20d,1次/d。并于第8、10、12天,除正常对照组外,均腹腔注射(ip)CTX 80mg/kg造成小鼠免疫抑制模型。测定并比较各组小鼠体质量增量、免疫器官指数、白细胞(WBC)数目、迟发型超敏反应(DTH)程度及巨噬细胞吞噬功能的差异。结果与模型组比较,一定剂量的甜牛大力和苦牛大力均可明显提高模型小鼠的WBC(P<0.01或P<0.05)数目;促进模型小鼠的DTH程度(P<0.01);增加模型小鼠的单核巨噬细胞的吞噬功能(P<0.01或P<0.05);不同程度提高模型小鼠的体质量、脾脏指数及胸腺指数(P<0.01或P<0.05)。其中,甜牛大力高、中剂量组(20、10g/kg)在提高胸腺指数和DTH程度方面,稍优于苦牛大力对应剂量组(P<0.05或P<0.01),而在其余指标上,甜牛大力与苦牛大力的作用差异无统计学意义(P>0.05)。结论甜牛大力和苦牛大力对CTX免疫抑制小鼠均具有一定的免疫功能增强作用,其中甜牛大力提高细胞免疫能力稍强于苦牛大力。
Objective To compare the immunoregulatory effects of Radix Millettia Speciosa （RM Speciosa） and Radix Millettia Championi （RM Championi） on immunosuppressed mice. Methods Kunming mice were randomly divided into normal control group, CTX model group, LMS positive group, RM Speciosa groups and RM Championi groups （20, 10 and 5 g/kg）. The mice were treated respectively with drug or NS once a day for consecutive 20 days. Mice in the other groups were injected intraperitoneally with CTX at days 8, 10 and 12 to establish immunosuppressed mice model except the normal group. The changes of body weight, immune organ weight, white blood cell （WBC） number, carbon particle clearance capability of maerophages and delayed type hypersensitivity （DTH） of mice in all groups were determined and compared. Results Compared with that in CTX group, the WBC number was significantly increased （P〈0.01 or P〈0.05） and the degree of DTH （P〈0.01） was enhanced at different doses in RM Speciosa groups and RM Championi groups. The activity of macrophages and the index of thymus and spleen were also improved in the treatment groups （P〈0.05 or P〈0.01）. The index of thymus and the degree of DTH in RM Speciosa groups （20 and 10 g/kg） were slightly higher than those in the corresponding RM Chamoioni groups （P〈0. 01 or P〈0.05）. For other indicators, RM Speciosa groups did not significantly differ from RM Championi groups （P〉0.05）. Oonclusion Both RM Speciosa and RM Championi can improve the immune function of CTX-induced immunosuppressed mice, and RM Speciosa is slightly superior to RM Championi in improving specific cellular immunity.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2016年第3期451-454,459,共5页
Journal of Xi’an Jiaotong University（Medical Sciences）
关键词
甜牛大力
苦牛大力
免疫调节功能
环磷酰胺
免疫抑制
Radix Millettia Speciosa （RM Speciosa）
Radix Millettia Championi （RM Championi）
immuno-regulation function
cyclophosphamide
immunosuppression
