摘要
为了构建真核表达载体pEGFP-FSH-C2-α和pEGFP-FSH-C2-β,并检测其在籽鹅颗粒细胞中的瞬时表达。利用PCR技术合成籽鹅FSH全基因后,构建促卵泡素α、β亚基真核表达载体,并转染籽鹅卵泡颗粒细胞。结果表明:基因片段已正确插入真核表达载体,同时在颗粒细胞中检测到pEGFP-FSH-C2-α和pEGFP-FSH-C2-β的融合蛋白表达。说明已构建真核表达载体pEGFP-FSH-C2-α和pEGFP-FSH-C2-β,并在颗粒细胞瞬时表达成功,为研究和开发生物制剂奠定基础。
To construct an eukaryotic expression vector of pEGFP-FSH-C2-α and pEGFP-FSH-C2-β and to investigate its expression in granulosa cells by Zi geese,the PCR technique was used to compound whole-genome of FSH by Zi geese based on the success of express in FSH whole-genome and cloning vectors in zi geese and to construct subunit of follitropin α,β eukaryotic expression vector,and the granulosa cells were transfected to ovarian follicle by Zi geese.The results showed that the gene fragment successfully inserted into the eukaryotic expression vector,and detected the fusion protein expression of pEGFP-FSH-C2-α and pEGFP-FSH-C2-βin granulosa cells.The eukaryotic expression vector pEGFP-FSH-C2-α and pEGFP-FSH-C2-β had been successfully constructed,and it could be expressed transiently in granulosa cells in order to lay a foundation to research and development biologics.
出处
《黑龙江八一农垦大学学报》
2015年第4期39-41,53,共4页
journal of heilongjiang bayi agricultural university
基金
黑龙江省农垦总局课题(HNKXIV-08-10a)
关键词
籽鹅
α-亚基
β-亚基
双酶切
颗粒细胞
Zi geese
α-subunit
β-subunit
double digestion
granulosa cells
