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山羊FSH真核表达载体pVITRO-FSHαβ构建及在CHO细胞内稳定表达 被引量：2

Construction and Stable Expression of Eukaryotic Expression Vector pVITRO-FSHαβ of Goat Follicle-stimulating Hormone in Chinese Hamster Ovary Cells(CHO)
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摘要 在对山羊促卵泡素α、β亚基基因克隆和瞬时表达的基础上,构建促卵泡素αβ双表达载体,并转染中国仓鼠卵巢细胞(Chinese Hamster Ovary Cells,CHO),在潮霉素B的抗性筛选下,进行稳定表达,获得了稳定表达的CHO细胞株,为重组激素制剂的研制提供了必备条件。 The dual expression vector pVITRO-FSHαβ was successfully constructed on the basis of cloning of goat FSH α＆β and transient expression. The plasmids were transfected into Chinese Hamster Ovary （CHO） cells, and stable expressing cells were acquired by screening of Hygromycin B. These results would make preparations for producing recombinant hormone.
作者 王建武 陈秀萍 严正杰 丁家桐
机构地区 扬州大学动物科学与技术学院
出处 《畜牧兽医学报》 CAS CSCD 北大核心 2005年第11期1130-1134,共5页 ACTA VETERINARIA ET ZOOTECHNICA SINICA
关键词 山羊促卵泡素 双表达载体 CHO 稳定表达 goat follicle-stimulating hormone dual expression vector CHO stable expression
分类号 S827.2 [农业科学—畜牧学]
  • 相关文献

参考文献18

  • 1Rose M P, Gaines Das R E, Balen A H. Definitionand measurement of follicle stimulating hormone[J].Endocrine Reviews, 2000,21 ( 1 ):5 - 22.
  • 2Boze H, C61ine L, Patrick C, et al. High-level secretory production of recombinant porcine follicle-stimulating hormone by pichia pastoris[J]. Process Biochemistry, 2001,36:907-913.
  • 3Coulibaly S, Besenfelder U, Miller I, et al. Expression and characterization of functional recombinant bovine follicle-stimulating hormone (boFSHα/β) produced in the milk of transgenic rabbits[J]. Molecular Reproduction and Development, 2002, 63 (3):300-308.
  • 4Dirnberger D, Steinkellner H, Abdennebi L, et al.Secretion of biologically active glycoforms of bovine follicle stimulating hormone in plants[J]. Eur J Biochem, 2001,268:4 570-4 579.
  • 5Fidler A E, Lun S, Young W, et al. Expression and secretion of a biologically active glycoprotein hormone,ovine follicle stimulating hormone, by Pichia pastoris[J]. Journal of Molecular Endocrinology, 1998, 21:327-336.
  • 6Keene J L, Matzuk M M, Otani T, et al. Expression of biologically active human follitropin in Chinese hamster ovary cells[J]. The Journal of Biological Chemistry,1989,264(9):4769-4 775.
  • 7范俊,邓晓惠.卵泡刺激素研究进展[J].生殖与避孕,1998,18(2):122-125. 被引量：13
  • 8丁家桐,张军强,孙怀昌,姜勋平.山羊促卵泡激素α和β亚单位cDNA的克隆与序列分析[J].农业生物技术学报,2003,11(4):432-434. 被引量：3
  • 9Kozak M. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs[J]. Nucleic Acids Research, 1987,15:8 125-8 148.
  • 10Kozak M. Initiation of translation in prokaryotes and eukaryotes[J]. Gene, J999,234:187-208.

二级参考文献22

  • 1Pierce J G, Parsous T F. Glycoprotein hormones, structure and function. Annu Rev Biochem, 1981, 50:465-495.
  • 2Ryan R J, Kertmann H T, Charlesworth M C, et al.Structure-function relationships of gonadotrophins, Recent Progress Hormone Res, 1987, 43:383-429.
  • 3Hoomans E H M, Andersen A N. A prospective randomized clinical trial comparing 150IU recombinant follicle stimulating hormone and 225IU highly purified urinary follicle stimulating hormone in a fixed dosed regimen in woman undergoing ovarian stimulation. Hum Repro, 1999,14:2442-2447.
  • 4Bello P A, Mountfor P S, Brandon M R, et al. Cloning and DNA sequence analysis of the cDNA for the common alpha-subunit of the ovine pituitary glycoprotein hormones.Nucl Acid Res, 1989, 17:10494.
  • 5Mountford P S, Bello P A, Brandon M R, et al. Cloning and DNA sequence analysis of the cDNA for the precursor of ovine follicle stimulating hormone beta-subunit. Nucl Acid Res, 1989,17:6391.
  • 6Esch F, Mason A, Cooksey K. Cloning and DNA sequence analysis of the eDNA for the precursor of the β chain of bovine follicle stimulating hormone. Proc Natl Acal Sci USA, 1986, 83:6619-6621.
  • 7Kato Y. Cloning and DNA sequence analysis of the cDNA for the precursor of pocrine follicle stimulating hormone (FSH) β subunit. Mol Cell Endocrinol, 1988, 55:107-112.
  • 8Erwin C R, Croyle M L, Donelson J E, et al. Nucleotide sequence of cloned complementary deoxyribonucleic acid for the α-subunit of bov-ine pituitary glycoprotein hormones.Biochemistry, 1983, 22:4856-4860.
  • 9刘殿芹，国外医学.计划生育分册，1996年，15卷，2期，124页
  • 10周清，国外医学.计划生育分册，1996年，15卷，3期，174页

共引文献136

同被引文献16

  • 1严正杰,孙雪萍,丁家桐.重组促卵泡素及长效类似物的研究进展[J].中国畜牧兽医,2005,32(5):12-14. 被引量：5
  • 2艾秀莲,陈新国,季青,龙涛,余红,昔奋攻,董平,石玉瑚.利用生物技术生产牛促卵激素的研究[J].中国科学（B辑）,1995,25(6):604-609. 被引量：10
  • 3严正杰,孙雪萍,丁家桐.长效山羊促卵泡素类似物真核表达载体的构建及其在CHO细胞内的稳定表达[J].生物技术通讯,2005,16(4):381-383. 被引量：2
  • 4孙淑斌,衣艳君,张培玉.人尿促性腺激素中卵泡刺激素的分离与纯化[J].曲阜师范大学学报（自然科学版）,1996,22(4):89-92. 被引量：3
  • 5Ryan R J, Charlesworth M C, McCormick D J, et al. The glycoprotein hormones: recent studies of sturcture-fuction relationships[J]. FASEB, 1988,2: 2661-2669.
  • 6Vicenta G C, Irving B. Novel recombinant gonadotropins[J]. Trends in Endocrinology and Metabolism, 2001, 12(2):72.
  • 7Sugahara T, Grootenhuis P D, Sato A, et al. Expression of biologically active fusion genes encoding the common α subunit and either the HCGβ or FSHβ subunits: role of a linker sequence[J]. Molecular and Cellular Endocrinology, 1996,125: 71-77.
  • 8Kozak M. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs[J]. Nucleic Acids Research, 1987,15:8125-8148.
  • 9Klein J, Lobe L. Development and characterization of a long-acting recombinant hFSH agonist[J]. Human Reproduction, 2003,18(1):50-56.
  • 10Mclachlan R I, Wreford N G, O'Donnell L, et al. The endocrine regulation of spermatogenesis: independent roles for testosterone and FSH[J]. Endocrinol, 1996,148:1-9.

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畜牧兽医学报
2005年 第11期

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