摘要
将重组有北京鸭抑制素α-亚基基因的T载体和pET-28a表达载体分别用NdeⅠ和XhoⅠ双酶切后连接并转化E.coli BL21(DE3),经PCR和双酶切鉴定,筛选得到阳性重组表达载体,在IPTG诱导下融合表达了北京鸭抑制素α-亚基蛋白。试验结果表明,抑制素α-亚基基因在E.coli BL21中得到了有效表达,表达产物具有免疫活性,为后期探讨该免疫原提高北京鸭产蛋性能的研究奠定基础。
pMD18-T vector with the inhibin msubunit gene from Beijing duck and pET-28a expression vector were cut by Nde I and Xho I . The products were connected and transformated into E. coli BL21 （DE3）. The positive recombinant ex- pression vectors were identificated by PCR and double digestion experiment. The inhibin α-subunit protein was induced by the IPTG. Tests in mice showed that inhibin α-subunit expressed effectively in E. coli BL21 had immunological competence. The results are helpful for the later research about improving the laving oerformanee of Beiiing duck.
出处
《中国畜牧兽医》
CAS
北大核心
2012年第7期134-137,共4页
China Animal Husbandry & Veterinary Medicine
基金
北京农业职业学院技术攻关与重点支持基金项目资助(yjxyf09-18)
