摘要
目的观察酪酪肽(PYY)对体外培养的胰腺癌Miapaca-2细胞凋亡的影响。方法不同浓度(0.01、0.005、0.002 5、0.001 25 mmol/L)PYY分别作用于体外常规培养的M iapaca-2细胞48 h后,流式细胞仪检测细胞凋亡率、细胞周期;RT-PCR法检测Survivin mRNA,Western blotting法检测Survivin、Caspase-3蛋白的表达。结果PYY处理48 h后细胞凋亡率明显升高,并呈浓度依赖性;G_0/G_1期细胞数明显增多,S、G_2/M期细胞数明显减少,G_1期前出现亚二倍体凋亡峰;PYY明显抑制Survivin蛋白和mRNA表达(P<0.01),上调Caspase-3蛋白表达(P<0.01)。结论 PYY能诱导胰腺癌Miapaca-2细胞凋亡,参与细胞周期调控,其机制可能与抑制Survivin蛋白和mRNA表达、上调Caspase-3蛋白表达有关。
Objectives To observe the effects of peptide YY（ PYY） on inducing apoptosis of human pancreatic cancer cell line Miapaca-2. Methods The pancreatic cancer cell line Miapaca-2 was treated with PYY of different concentrations（ 0. 01,0. 00 5,0. 002 5 and 0. 001 25 mmol / L） for 48 h. The apoptosis and cell cycle were evaluated by flowcytometry,the expression of survivin mRNA was detected with RT-PCR,and the expressions of survivin and Caspase-3 protein were detected with Western blotting. Results After intervention with PYY for 48 h,celluar apoptosis rate was significantly increased in a dose-dependent manner. The cells of G0/ G1 stage were significantly increased,but the cells of S and G2/ Mstages were significantly decreased. The hypodiploid apoptotic peak before the G1 stage was detected. PYY inhibited survivin protein and survivin mRNA expressions but increased Caspase-3 protein expression notably in a dose dependent manner（ P〈0. 01）. Conclusion PYY can induce apoptosis in a dose-dependent manner.PYY maybe block cell cyclein G0/ G1 stage,downregulate survivin mRNA and protein expressions,increase Caspase-3protein expression and finally induce apoptosis.
出处
《山东大学学报（医学版）》
CAS
北大核心
2015年第12期7-11,共5页
Journal of Shandong University:Health Sciences
基金
国家自然科学基金(81170352)
