摘要
目的:探讨半枝莲提取物野黄芩苷对人乳腺癌细胞系MCF-7的抑制作用及机制。方法:用不同浓度野黄芩苷处理人乳腺癌细胞系MCF-7。MTT比色实验观察其对人乳腺癌细胞系MCF-7增殖的抑制作用。选择合适的药物浓度及培养时间处理人乳腺癌细胞MCF-7后依次进行提取标本总RNA。采用miRNA生物芯片微阵列基因表达和分析技术筛选差异表达的miRNA,实时荧光定量PCR技术对其中有明显差异性表达的miRNA进行验证。结果:野黄芩苷在一定浓度范围内能抑制人乳腺癌细胞系MCF-7的增殖。野黄芩苷能显著上调miR-15a、miR-16的表达。结论:野黄芩苷能抑制人乳腺癌细胞系MCF-7的增殖,并上调miRNA-15a、miRNA-16的表达。
Objective： To explore the inhibitory effect and mechanism of scutellarin on human breast cancer cell line MCF- 7. Methods： Human breast cancer cells line MCF- 7 was treated with different concentrations of scutellarin. The proliferation on human breast cancer cells line MCF- 7 was determined by MTT colorimetric experimental.The appropriate drug concentration and incubation time were chosen to deal with human breast cancer cells line MCF- 7,and then,the RNA was extracted. The microarray gene expression and analysis technology were used to screen the differentially expression of miRNAs and the real- time fluorescence quantitative PCR technology was used to validate the differences expression of miRNAs. Results： In the certain concentration range,scutellarin can inhibit the proliferation of human breast cancer cells line MCF- 7. Scutellarin can significantly increase the expression of the miR-15 a and miR- 16. Conclusion： Scutellarin can inhibit the proliferation of human breast cancer cells line MCF- 7,and the up regulate the miRNA- 15 a and miRNA- 16.
出处
《现代肿瘤医学》
CAS
2016年第2期197-200,共4页
Journal of Modern Oncology
基金
黑龙江省教育厅科学技术研究项目(编号:12521245)
