摘要
目的:探讨半枝莲对大鼠原发性肝癌(primary liver cancer,PLC)组织中去整合素金属蛋白酶10(a disintegrin and metalloproteinase 10,ADAM10)表达的影响。方法:120只Wistar大鼠随机分为对照组、模型组、苦参碱组和半枝莲组,以二乙基亚硝胺诱发PLC大鼠模型(对照组除外),分别给予半枝莲组和苦参碱组大鼠相应药物灌胃,对照组和模型组大鼠给予等量0.9%氯化钠溶液灌胃。计数各组大鼠肝脏表面的癌结节数,并计算抑瘤率;苏木精-伊红染色观察各组大鼠肝脏组织的病理学改变。免疫组织化学法和蛋白质印迹法检测对照组大鼠肝脏组织及模型组、苦参碱组和半枝莲组大鼠肝癌组织中ADAM10的表达。结果:苦参碱组和半枝莲组大鼠的肝癌结节数明显少于模型组(P<0.05),并出现凋亡小体;半枝莲组的抑瘤率显著高于苦参碱组(P<0.05)。模型组肝癌组织中ADAM10的阳性表达率及蛋白表达水平显著高于对照组肝脏组织(P<0.05),半枝莲组和苦参碱组肝癌组织中ADAM10的阳性表达率和蛋白质表达水平显著低于模型组(P<0.05),半枝莲组肝癌组织中ADAM10的阳性表达率和蛋白质表达水平显著低于苦参碱组(P<0.05)。结论:半枝莲可能通过下调ADAM10的表达,抑制大鼠肝癌的形成。
Objective： To investigate the effect of Scutellaria barbata D. Don on the expression of ADAM10 （a disintegrin and metalloproteinase 10） in primary liver cancer tissues in rats. Methods： Total of 120 Wistar rats were randomly divided into four groups： normal, model, matrine and Scutellaria barbata D. Don groups. The model of rat bearing primary liver cancer was induced by diethylnitrosamine. The rats in the matrine group and Scutellaria barbata D. Don group were fed with matrine and Scutellaria barbata D. Don powder, respectively. The rats in the normal group and model group were fed with 0.9% sodium chloride solution. The number of hepatocellular carcinoma nodules was calculated, and the tumor growth inhibition rate was also calculated. The pathologic changes of hepatic tissues in rats of each group were observed by hematoxylin and eosin staining. The expressions of ADAM10 in liver cancer tissues in these four groups were determined by immunohistochemistry and Western boltting. Results： The number of hepatocellular carcinoma nodules of the matrine group and Scutellaria barbata D. Don group was less than that of the model group （P 〈 0.05） with the appearance of apoptotic bodies. The tumor growth inhibition rate of Scutellaria barbata D. Don group was higher than that of the matrine group （P 〈 0.05）. The positive expression rate and the expression level of ADAM10 in liver cancer tissues of the model group were significantly higher than those of the normal group （P 〈 0.05）. The positive expression rate and the expression level of ADAM10 in liver cancer tissues of matrine group and Scutellaria barbata D. Don group were lower than those of the model group （P 〈 0.05）. The positive expression rate andthe expression level of ADAM10 in liver cancer tissues of Scutellaria barbata D. Don group were lower than those of matrine group （P 〈 0.05）. Conclusion： Scutellaria barbata D. Don may inhibit the growth of primary liver cancer in rats through down-regulating the expression of ADAM10.
出处
《肿瘤》
CAS
CSCD
北大核心
2013年第10期879-883,共5页
Tumor
