摘要
目的利用微卫星分子标记技术对杜仲居群遗传多样性及亲缘关系进行研究。方法对设计的29对EST-SSR引物以及20对已发表的杜仲SSR引物进行筛选,获得的高多态性引物对不同产区杜仲的125个个体进行遗传多样性及亲缘关系分析。结果筛选得到的18对引物共扩增出74条清晰带。Nei's基因多样性指数H=0.3750,Shannon's多态性信息指数I=0.5512。基因分化系数Gst=0.1149。湖南石门与桑植之间有一定的亲缘关系,重庆秀山与陕西略阳之间遗传距离最大。结论杜仲居群内具有丰富的遗传多样性,而不同群体间的遗传多样性低,居群间存在较大基因流。
Objective To investigate the genetic diversity and relationships of Eucommia ulmoides using simple sequence repeat（ SSR） markers. Methods 29 pairs of EST- SSR primers and 20 pairs published SSR primers were screened. The genetic diversity and relationships of 125 individuals for different areas were analyzed using highly polymorphic primers. Results The 18 pairs of highly polymorphic primers were screened and 74 clear bands were amplified totally. Nei＇s genetic diversity index H = 0. 3750,Shannon＇s information index polymorphism I = 0. 5512. Gene differentiation coefficient Gst = 0. 1149. Eucommia ulmoides from Shimen showed a closer systematic relationship with Eucommia ulmoides from Sangzhi. The genetic distance was the largest between Chongqing and Lueyang. Conclusion Eucommia ulmoides populations have genetic diversity and low genetic diversity among different populations. The results showed that there is a larger gene flow between the populations.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2015年第10期2507-2509,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家科技支撑计划课题(No.2011BAI01B08)
湖南省中医药研究院院基金项目
