摘要
为了促进千里香种质资源的保护利用,为千里香新品种选育提供依据,该文对千里香种质资源进行遗传多样性分析,并构建了DNA分子身份证。基于千里香基因组筛选出的9个SSR标记,以65份千里香种质为材料,采用多重荧光毛细管电泳检测技术分析扩增条带相对分子质量和等位基因。根据9对SSR引物的扩增带型,对每个千里香种质材料进行遗传多样性分析,并构建供试样品的DNA分子身份证。结果表明,9对SSR引物共检测出78个等位基因,平均等位基因数为8.67个;观测杂合度为0.338~0.831,平均为0.601;期望杂合度为0.413~0.853,平均为0.721;Shannon′s信息指数为0.880~1.994,平均为1.41;多态性信息含量为0.391~0.835,平均为0.696,遗传多样性丰富。当遗传相似系数为0.347时,可将65个种质划分为5个群组。在此基础上,利用多态性较高的5对核心引物组合,构建了千里香种质DNA分子身份证,并为云浮种质资源圃中保存的49份核心种质制作了二维码身份证,为千里香的新品种选育、生产利用及溯源奠定基础。
To promote the conservation and utilization of the germplasm resources and provide a basis for the breeding of new varieties of Murraya paniculata,this study analyzed the genetic diversity of the germplasm resources and developed the molecular identity(ID)card of M.paniculata.Multiple fluorescence PCR-capillary electrophoresis was performed for 65 germplasm accessions of M.paniculata based on 9 SSR markers identified from the M.paniculata genome,and the molecular weights and alleles of the amplified bands were analyzed.According to the banding patterns of the 9 SSR primers,this study analyzed the genetic diversity of each germplasm accession of M.paniculata and developed molecular ID cards for the test samples.The results showed that 9 pairs of SSR primers detected 78 alleles,with an average of 8.67 alleles.The observed and expected heterozygosity was 0.338–0.831(average of 0.601)and 0.413–0.853(average of 0.721),respectively.The Shannon’s information index varied within the range of 0.880–1.994,with an average of 1.41.The polymorphic information content was within the range of 0.391–0.835,with an average of 0.696,which indicated rich genetic diversity.When the genetic identity was 0.347,the 65 germplasm accessions were classified into 5 groups.Based on the results,this study employed the 5 SSR primers with higher polymorphisms to develop the molecular ID cards for the germplasm resources of M.paniculata and created QR code ID cards for the 49 core germplasm accessions preserved in the Yunfu germplasm nursery,laying a foundation for the new variety breeding,production,utilization,and traceability of M.paniculata.
作者
孙诚
王博铖
陈梓媛
蒋超
谢文波
袁媛
SUN Cheng;WANG Bo-cheng;CHEN Zi-yuan;JIANG Chao;XIE Wen-bo;YUAN Yuan(Guangdong Pharmaceutical University,Guangzhou 510006,China;Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China;State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;China Resources Sanjiu Medical&Pharmaceutical Co.,Ltd.,Shenzhen 518029,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2024年第23期6272-6280,共9页
China Journal of Chinese Materia Medica
基金
中国中医科学院科技创新工程重大攻关项目(CI2021A041,CI2021B014,CI2023D001,CI2023E002-04)
国家自然科学基金项目(82325049)
中央本级重大增减支项目(2060302)。
