摘要
探讨新型类嘌呤脱氧核糖核苷类化合物体外抗乙型肝炎病毒(HBV)的作用,为了将新型类嘌呤脱氧核糖核苷类化合物作用于以Hep G2.2.15细胞系,通过四甲基偶氮唑盐(MTT)比色法检测样品对Hep G2.2.15细胞的毒性,采用酶联免疫吸附测定技术(ELISA)检测细胞上清中HBsAg和HBeAg的变化.结果显示,新型类嘌呤脱氧核糖核苷类化合物在第9天时,浓度为10μmol/L,对HBsAg和HBeAg的抑制率都较高,化合物的毒性也较低.体外细胞培养表明,新型类嘌呤脱氧核糖核苷类化合物在体外有一定的抗乙型肝炎病毒作用.
To investigate the effect of anti-HBV of novel purine deoxy nucleoside ribose analogues in vitro . The levels of HBsAg or HBeAg in the cultured supermatant of HepG 2.2.15 were tested by ELISA.The cell vitality also was tested with MTT method for evaluating the cytotoxic effect of novel purine deoxy nucleoside ribose analogues. After 9 d,novel purine deoxy nucleoside ribose analogues showed low cytotoxic effect on 2.2.15 cel1 in the concentration under 10 μmol/L. The inhibitive rates were high for HBsAg and HBeAg. Novel purine deoxy nucleoside ribose analogues show the anti-HBV activity in HepG 2.2.15 cell lined cultured in vitro.
出处
《河南科学》
2015年第6期911-914,共4页
Henan Science
基金
河南省科技创新杰出青年基金(094100510019)
河南省科技攻关计划资助项目(092102310021)
