摘要
目的:探讨藤茶双氢杨梅树皮素(DHM)在2215细胞培养中对乙肝病毒HBsAg、HBeAg分泌的抑制作用。方法:通过DHM含药血清对2215细胞进行毒性实验,在最佳DHM含药血清下,测定HB-sAg和HBeAg水平。结果:DHM含药血清的最佳添加量为不超过20%;在最佳添加量不超过20%浓度下,DHM对2215细胞HBsAg、HBeAg的分泌有明显的抑制作用。结论:DHM可明显抑制2215细胞HBsAg和HBeAg的分泌。
Objective： To explore the effect of DHM of ampelopsis grossedentata on the inhibition of 2215 cell secretion of HBsAg and HBeAg. Methods： The toxicity experiment of 2215 cell was performed using serum with DHM, the level of HBsAg and HBeAg was determined on optimal concentration of DHM. Results： The optimal concentration of DHM in serum was no more than 20%; with which the DHM could inhibit effectively the secretion of HBsAg and HBeAg in 2215 cell. Conclusion： DHM can inhibit markedly the secretion of HBsAg and HBeAg of 2215 cell.
出处
《海南医学院学报》
CAS
2008年第3期212-214,共3页
Journal of Hainan Medical University
关键词
肝炎病毒
乙型
植物
药用
细胞培养
酶联免疫吸附测定
Hepatic virus, type B
Vegetable, medicine
Cell culture
Enzyme linked immunosorbent assay
