摘要
以北碚榕茎尖（1.5 cm）为外植体,通过丛芽诱导、增殖和生根建立离体繁殖体系。结果表明,茎尖在MS＋1.5 mg·L-16-BA＋0.05 mg·L-1 NAA培养基中培养30 d,丛芽诱导率达100%,繁殖系数达9.2；不定芽在此培养基中继代培养,1~6代平均繁殖系数达7.0,丛芽长势良好；不定芽在1/2MS培养基中,30 d生根率达100%,根系发达,试管苗生长健壮。105株试管苗移栽到土壤营养钵中,30 d存活102株,成活率达97.14%。1个茎尖外植体在半年内可繁殖试管苗10万株。
The stem tips （1.5 cm） of Ficus beipeiensis were used as explants to establish a propagation system in vitro. The results indicated that the stem tip explants were cultured on MS medium supplemented with 1.5 mg·L-1 6-BA＋0.05 mg·L-1 NAA for 30 days, the induction rate of the adventitious buds achieved 100%and a propagation coefifcient reached 9.2. The adventitious buds were subcultured every other 30 day. The Average multiplication coefifcient achieved 7.0 from 1 to 6 generations, and the cluster buds grew well. The adventitious buds were trained on 1/2MS basal medium for 30 d, their rooting rate was as high as 100%, the root system de-veloped well and the test-tube plantlets grew vigorously. One hundred and ifve rooted plantlets were then trans-planted to matrix soil, and 102 of them （97.14%） survived after 30 days’ culture. A stem tip can multiply one hundred thousand of test-tube plantlets in six mouths.
出处
《植物生理学报》
CAS
CSCD
北大核心
2015年第4期471-475,共5页
Plant Physiology Journal
基金
国家自然科学基金(31370317)
三峡库区生态环境教育部重点实验室开放基金(SXKQSYS-ZW-2013001)
关键词
北碚榕
茎尖
组织培养
快速繁殖
Ficus beipeiensis
stem tip
tissue culture
rapid propagation
