摘要
目的:探讨人颊癌细胞中细胞因子信号转导抑制因子1(SOCS1)沉默与细胞增殖及化疗药物敏感性影响的关系。方法:Western blot、PCR及定量PCR验证SOCS1干扰序列沉默人颊癌细胞系BcaC D885中SOCS1的表达。MTT法检测颊癌细胞对化疗药物敏感性的变化。细胞计数法观察细胞的增殖速度。流式细胞术检测细胞周期的变化。结果:将SOCS1干扰序列转染BcaC D885细胞后,SOCS1 mRNA及蛋白表达水平均明显下降。SOCS1表达抑制后,转染72h后,BcaC D885细胞数量较对照组显著减少(P<0.05)。化疗药物卡铂和紫杉醇对BcaC D885细胞的半数抑制浓度(IC50)均显著降低(P<0.01)。SOCS1表达抑制后BcaC D885细胞G0/G1期细胞比例明显升高,而S期和G2/M期细胞比例明显降低,与对照组比较,差异均具有统计学意义(P<0.05)。结论:SOCS1基因沉默后,人颊癌细胞株BcaC D885的增殖能力下降,并增强对化疗药物的敏感性。
Objective：To detect the changes of cell proliferation and chemotherapeutic drugs sensitivity of human carcinoma of the buccal mucosa after suppressor of cytokine signaling - 1 （SOCS1） gene silencing. Methods：Western blot,PCR and real -time PCR were used to verify the down regulation of SOCS1 human carcinoma of the buccal mu- cosa cell （BcaCD885） after transfection, and the change of chemotherapeutic drugs sensitivity was detected by MTT assay, cell counting was used to measure cell proliferation rate, flow cytometry was used to detect the cell cycle. Re- suits： Results of RT - PCR and Western blot showed that the expression levels of SOCS1 mRNA and protein were sig- nificantly decreased in small hairpin SOCSI （shSOCS1） transfected BcaCD885 cells. After the silence of SOCS1 ,the median inhibitory concentration of chemotherapeutic drugs for BcaCD885 ceils decreased significantly （ P 〈 0.01 ）. 72 hours after the SOCS1 silencing,the cell count of shSOCS1 cells dropped significantly, compared with that of control group （P 〈 0.05）. The cell cycle arrest was promoted at the G_0/G_1 phase, but the percentage of cells in S phase and GJM decreased compared to that of control groups （ P 〈 0.05 ）. Conclusion： Inhibition of SOCS1 gene expression de- creases the proliferation ability of human carcinoma of the buccal mucosa cell line BcaCD885, and enhances the sensi- tivity of BcaCD885 cells to chemotherapeutic drugs.
出处
《现代肿瘤医学》
CAS
2015年第8期1053-1056,共4页
Journal of Modern Oncology
关键词
人颊癌
细胞因子信号转导蛋白抑制因子
基因沉默
细胞增殖
human carcinoma of the buccal mucosa
suppressor of cytokine signaling proteins
gene silencing
cellproliferation
