摘要
目的:研究细胞信号转导抑制因子1(SOCS1)基因对人口腔癌细胞增殖、周期及体外侵袭生物学功能的影响。方法:Western blotting及实时定量PCR验证人口腔癌细胞系KB中基因SOCS1的干扰效果;MTT法检测细胞增殖;应用Transwell侵袭小室模型观察口腔癌细胞的侵袭能力;流式细胞术分析细胞周期的分布情况。结果:SOCS1干扰片段显著降低口腔癌KB细胞SOCS1基因的m RNA及蛋白表达水平。抑制SOCS1表达后,细胞体外侵袭能力明显下降(P<0.05),且转染72 h后KB细胞数量较对照组显著减少(P<0.05);干扰KB细胞SOCS1基因表达,使G0/G1期细胞数目明显多于对照组(P<0.05),而S、G2/M期细胞数目显著少于对照组(均P<0.05)。结论:干扰人口腔癌细胞株KB中的SOCS1基因表达后,KB细胞增殖、体外侵袭能力减弱,细胞分裂受到抑制。
Objective：To investigate the effects of cell proliferation,cell cycle and invasion ability of human oral cancer cell after suppressor of cytokine signaling-1（SOCS1） gene interference. Method：Western blotting,real-time PCR were used to verify the down regulation of SOCS1 human oral cancer cell line（KB） after transfection;and the cell proliferation rate was detected by MTT assay;the invasive ability of KB cells in vitro was evaluated by Transwell migration assay;flow cytometry was used to detect the cell cycle. Result：Results of RT-PCR and western blotting showed that the expression levels of SOCS1 m RNA and protein were significantly decreased in small interfering SOCS1 transfected KB cells. 72 hours after the SOCS1 silencing,the cell proliferation rate dropped significantly compared with that of control group（P〈0.05）.After the silence of SOCS1,the invasive ability of KB cells in vitro was decreased（P〈0.05）. The cell cycle arrest was promoted at the G0 / G1 phase,but the percentage of cells in S phase and G2 / M decreased compared to that of control groups（P〈0.05）. Conclusion：Inhibition of SOCS1 gene expression decreases the proliferation ability,and invasion ability in vitro of human oral cancer cell line KB,and inhibits cell division.
出处
《临床口腔医学杂志》
2016年第5期259-261,共3页
Journal of Clinical Stomatology
