摘要
目的:研究siRNA干扰β-catenin对人骨肉瘤U2OS细胞增殖能力的影响。方法:利用脂质体Lipofectamine 2000将靶向β-catenin的siRNA转染人骨肉瘤U2OS细胞,Western blot技术检测β-catenin基因沉默效果。分别采用CCK-8法和流式细胞学技术检测细胞增殖和周期,Western blot检测cyclin D1的蛋白表达。结果:siRNA干扰有效阻断了人骨肉瘤U2OS细胞的β-catenin表达。β-catenin siRNA转染U2OS细胞48h、72h后,与空白组和阴性对照组相比,细胞增殖能力显著下降,细胞周期明显阻滞于G0/G1期,细胞中cyclin D1蛋白表达水平明显下降(P<0.05)。结论:特异性抑制β-catenin基因表达可抑制人骨肉瘤U2OS细胞增殖。
Objective ：To investigate the effect ofβ -catenin silence by siRNA interference on the proliferation of human osteosarcoma cell U20S. Methods ：The β -eatenin -siRNA was transfected into U20S cells with Lipofectami- neTM 2000. Theβ- catenin expression was detected by Western blot after transfection to investigate the silence effect. Cell cycle was detected by flow cytometry, cell proliferation was assayed by CCK -8 assay and the protein expression of cyclin D1 was detected by Western blot. Results ： siRNA interference effectively block the expression of β - catenin in human osteosarcoma cell U20S. The cell proliferation was obviously decreased. Cell cycle analysis presented obvi- ous G0o/G1 blockage and the expression of cyclin D1 protein was significantly decreased in U2OS cells transfected withβ- catenin siRNA for 48h and 72h （ P 〈 0.05 ）. Conclusion： Specific blockage of β- catenin expression inhibited the proliferation of human osteosarcoma cell U2OS.
出处
《现代肿瘤医学》
CAS
2013年第12期2684-2687,共4页
Journal of Modern Oncology
基金
辽宁省自然科学基金(编号:20102218)
