摘要
从东北地区获得两类型卫氏并殖吸虫,提取其基因组DNA。用5种限制性内切酶消化,琼脂糖凝胶电泳后,观察两类型成虫基因组DNA的酶切片段。又用Southern印渍技术对两类型7种酶消化物的结果做了比较。显示两类型Bam HI、Hind Ⅲ及Eco RI的酶切结果基本相同,但Pst Ⅰ、DdeⅠ、HaeⅢ及HpaⅡ的结果却显示两类型的DNA重复顺序有明显差别。此结果在单个虫体DNA的杂交技术检测中也得到证实。
Restriction enzyme digestion of total genomic DNA of two chromosome forms of Paragonimus westermani showed the presence of homologous highly repeated DNA in both diploid and triploid forms. Southern blotting analysis provided further evidence that the distribution, of restriction enzyme sites (with 3 enzymes) on repetitive sequence of DNA of both forms were similar. However, with PstⅠ, Ddel, HaeⅢ and HpaⅡ, their polymorphism revealed differences which were also found in each form tested separately with the hybridization technique.The present study, at the molecular level, supports the previously reported biological and biochemical results that they might be considered as different isolates or forms.It is suggested that PstⅠ, DdeⅠ, HaeⅢ and HpaⅡdigestion pattern might be useful to distinguish the diploid from the triploid forms of Paragonimus westermani.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1991年第1期46-49,共4页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
并殖吸虫
基因组
DNA
染色体
Paragonimus westermani, DNA sequence, restriction endonuclease, southern blotting.
