摘要
本文报道卫氏并殖吸虫囊蚴和成虫可溶性抗原（ＰｗＭ和ＰｗＡ）ＳＤＳ－ＰＡＧＥ后银染色及相应单克隆抗体（ＭｃＡｂ）识别免疫酶染色的结果。银染色显示ＰｗＭ有２２条蛋白带，其中主带有１０条，分子量分别为１２～１３、１４、４０、４１、４２、４８、５６、５７、６３和８０ｋＤ。ＰｗＡ有４１条带，其中主带有２１条，分子量分别为１１～１２、１４、１６～１７、１８、１９、２１～２３、２４～２６、３３、３９、４０、４１、４２、４３～４７、４８、５１、５６、８７、９７和１６０～１６４ｋＤ，另外２条主带分子量在２００ｋＤ以上。针对ＰｗＭ的ＭｃＡｂＧ２Ｂ３与ＰｗＭ反应出现３条蛋白带，分子量分别为４２、４８、６３ｋＤ。针对ＰｗＡ的ＭｃＡｂＡ３Ｄ３与ＰｗＡ反应出现１２条蛋白带，分子量分别为２１～２３、５６、７０、８７、９７、９８、１０４、１２０、１２５、１６０～１６４ｋＤ，另外２条带分子量在２００ｋＤ以上。
The components of metacercariae and adult worms of Paragonimus westermani(PwM and PwA)were analysed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE),silver stain and western bloting. 22 and 41 protein bands were to be present inPwM and PwA respectively.The molecular weights of main bands were 12-13,14,16-17,18,19,21-23,24-26,33,40,41,42,43-47,48,51,56,87,97 and 160-164 kD(21 bands)in PwA.The vvestern blot examination of PwA with monoclonal antibodies against metacercariae( G2B3)and adult worms(A2D3 ) revealed 3 and 12 bands separately,these protein molecular weightswere 42,48, and 63 kD in PwM with G2B3;21-23,56,70,87,97,98,104,120’l25,160-164 and2 bands over 200 kD in PwA with A3D3.
出处
《中国寄生虫病防治杂志》
CSCD
1994年第4期266-269,共4页
Chinese Journal of Parasitic Disease Control
基金
自然科学基金
关键词
卫氏并殖吸虫
囊蚴
成虫
抗原
单克隆抗体
PwM and PwA SDS-PAGE silver stain western bloting monoclonalantibodies
