摘要
目的 分析浙江省舟山海岛地区抗核抗体和抗核抗体谱阳性分布趋势,及其在就诊人群中的诊断价值.方法 分别用间接免疫荧光法（IIF）和线性免疫印迹法（LIA）检测抗核抗体.资料分布的比较采用x2检验.结果 抗核抗体（ANA）阳性患者总阳性率为16.3％ （1886/11578）,女性阳性率（20.6％,431/6955）高于男性（9.8％,5/4623）,差异有统计学意义（x2=236.7,P＜0.05）.21 ～40岁组阳性率（1.5％,38/2036）显著高于其他年龄组,差异有统计学意义（x2=115.8,P＜0.05）.抗核抗体谱阳性患者2722例,阳性率为23.5％.抗核抗体谱中抗SS-A、抗Ro-52、抗着丝点抗体的阳性率最高,分别为31.7％、28.4％和8.3％.女性抗U1-nRNP、抗核糖体P蛋白、抗dsDNA等抗体的阳性率明显高于男性（P＜0.05）.不同年龄组的抗核抗体谱阳性率分析结果显示抗U1-nRNP、抗Sm、抗核糖体P蛋白等抗体的阳性率差异具有统计学意义（P＜0.05）.263例不同自身免疫性疾病组中抗核抗体谱阳性率的分析显示RA、SLE、SS、MCTD、PBC组阳性率最高的自身抗体分别是抗Ro-52抗体、抗SS-A抗体、抗SS-B抗体、抗U1-nRNP抗体和抗M-2抗体.结论 舟山海岛地区就诊人群中自身免疫性疾病好发于中年女性,在临床检测中应采用多种自身抗体的联合检测,以提高自身免疫性疾病的诊断率.
Objective To review the results of anti-nuclear antibodies （ANA） and explore the diagnose value for patients with related diseases.Methods Indirect immunofluorescence （IIF） and line immunoassay （LIA） methods were used to detect ANA and specific ANA spectrum respectively.Chi square test was applied to analyse the distributed data.Results The positive rate of whole patinets was 16.3％ （1886/11578）.The positive rate of female patients（20.6％,431/6955） was significantly higher than the male patients （9.8％,5/4623） （x2 =236.7,P ＜ 0.05）.The positive rate in 21 ～ 40 age group was significantly higher than the other age groups（x2 =115.8,P ＜ 0.05）.On the other hand,2722 cases got positive results in ANA-LIA tests,which gave a positive rate of 23.5 ％.Data in this study showed that higher positive rates found in all patients were anti-SS-A antibodies （31.7％）,anti-Ro-52 antibodies （28.4％） and anti-centromere antibodies （8.3％）.The positive rates of antibodies such as anti-U1-nRNP,anti-ribosomal P protein,anti-dsDNA in females were significantly higher than in males （P ＜ 0.05）.The positive rates of ANA-LIA tests such as anti-U1 -nRNP,anti-SM,anti-ribosomal P protein in all age groups were significantly different than each other（P ＜ 0.05）.The highest positive rates in 263 patients above with RA,SLE,SS,MCTD,PBC were antibodies against to Ro52,SS-A,SS-B,U1-nRNP and mitochondrial-M2 respectively.Conclusion In patients of Zhoushan islands,middle-aged women face higher risk of developing autoimmune diseases.It is necessary to diagnose the autoimmune diseases by joint detection of lots of antibodies.
出处
《医学研究杂志》
2014年第12期122-125,共4页
Journal of Medical Research
