摘要
建立了固相萃取法净化样品、高效液相色谱-三重四级杆质谱法（HPLC-MS/MS）同时测定面粉中17种真菌毒素的检测方法。样品以乙腈：水：乙酸（84：15：1）作为提取溶剂,利用强阴离子交换固相萃取柱进行净化浓缩,甲醇、乙酸铵和甲酸的水溶液作流动相进行梯度洗脱,以HPLCMS/MS测定面粉样品中赭曲霉毒素、烟曲霉素和黄曲霉毒素B1、B2等17种真菌毒素。结果表明：方法线性范围为0.5～50 ng/mL,样品回收率在78.65%～103.40%之间,相对标准偏差小于5%。方法能满足当前面粉中真菌毒素的检测要求。
A method has been developed for simultaneous determination of 17 mycotoxins in flour by solid phase extraction（SPE） and high performance liquid chromatography-tandem mass spectrometry（HPLCMS/MS）. The samples were sequentially extracted by acetonitrile：water：acetic acid（v/v=84：15：1）, then purified and concentrated by strong anion exchange solid phase extraction（SAE-SPE）, and determined with HPLC-MS/MS for 17 kinds of mycotoxins in flour samples using methanol, aqueous of ammonium acetate and formic acid as mobile phase with gradient elution. The linear range was 0.5～50 ng/mL. The average recovery rate in flour was in the range of 78.65%~103.40%, and the relative standard deviation（RSD） was less than 5%. This method can meet the current detection requirements for mycotoxins in flour.
出处
《食品科技》
CAS
北大核心
2014年第11期331-335,共5页
Food Science and Technology
基金
江苏省质量技术监督局项目(KJ133808)
